Camponeschi Ilaria, Montanari Arianna, Beccaccioli Marzia, Reverberi Massimo, Mazzoni Cristina, Bianchi Michele M
Department of Biology and Biotechnology 'C. Darwin', Sapienza University of Rome, Rome, Italy.
Department of Environmental Biology, Sapienza University of Rome, Rome, Italy.
Front Microbiol. 2021 Jul 14;12:705012. doi: 10.3389/fmicb.2021.705012. eCollection 2021.
In unicellular organisms like yeasts, which do not have specialized tissues for protection against environmental challenges, the presence of cellular mechanisms to respond and adapt to stress conditions is fundamental. In this work, we aimed to investigate the response to environmental light in . Yeast lacks specialized light-sensing proteins; however, has been reported to respond to light by increasing hydrogen peroxide level and triggering nuclear translocation of Msn2. This is a stress-sensitive transcription factor also present in . To investigate light response in this yeast, we analyzed the different phenotypes generated by the deletion of the hypoxia responsive and lipid biosynthesis transcription factor Mga2. Alterations in growth rate, mitochondrial functioning, ROS metabolism, and fatty acid biosynthesis provide evidence that light was a source of stress in and that Mga2 had a role in the light-stress response. The involvement of Msn2 and Crz1 in light stress was also explored, but the latter showed no function in this response.
在诸如酵母这类没有专门组织来抵御环境挑战的单细胞生物中，存在应对和适应应激条件的细胞机制至关重要。在这项工作中，我们旨在研究[具体酵母种类未给出]对环境光的反应。酵母缺乏专门的光感应蛋白；然而，据报道[具体酵母种类未给出]通过提高过氧化氢水平和触发Msn2的核转位来对光作出反应。这是一种在[具体酵母种类未给出]中也存在的应激敏感转录因子。为了研究这种酵母中的光反应，我们分析了缺氧反应和脂质生物合成转录因子Mga2缺失所产生的不同表型。生长速率、线粒体功能、活性氧代谢和脂肪酸生物合成的改变提供了证据，表明光在[具体酵母种类未给出]中是一种应激源，并且Mga2在光应激反应中发挥作用。我们还探究了Msn2和Crz1在光应激中的作用，但后者在这种反应中未显示出功能。
