School of Physical Education, Shanxi University, Taiyuan 030006, China.
Arba Minch University, Arba Minch, Ethiopia.
J Healthc Eng. 2021 Jul 21;2021:7600835. doi: 10.1155/2021/7600835. eCollection 2021.
The metabolic characteristics of body fluids of excellent water polo players before and after competition have not been reported. The purpose of the study was to compare the metabolites in the urine of water polo players before and after competition by H-NMR-based metabolomic approach.
Twenty-six young water polo players participated in the study voluntarily. The urine and blood samples of players were collected one week before competition (A), immediately after competition (B), and one week after competition (C). Metabolomic analysis was conducted on the urine samples. Urine routine items and biochemical indicators in blood samples were detected.
Metabolomic results showed that the contents of eleven metabolites including lactic acid, acetoacetate, and succinic acid in the urine of the subjects increased and four metabolites such as dimethylamine, choline, and glucose decreased at stage B. Most metabolites at stage C had basically returned to the levels at stage A. Pyruvate metabolism, pantothenate and CoA biosynthesis, synthesis, and degradation of ketone bodies were mainly involved in the above process. Urine conventional analysis results showed that the urine pH decreased dramatically and the levels of PRO and URO significantly increased at stage B, and the three indicators had similar values between stages A and C. The other indicators did not have obvious difference among the three stages. Analysis of blood biochemical indicators showed that the levels of LDH, BUN, CK, and AST significantly increased at stage B and did not show an obvious difference between stages A and C. The results are helpful for coaches to arrange the athletes' diet reasonably and to conduct scientific training for athletes.
优秀水球运动员比赛前后体液的代谢特征尚未报道。本研究旨在通过基于 H-NMR 的代谢组学方法比较水球运动员比赛前后尿液中的代谢物。
26 名年轻水球运动员自愿参加了这项研究。在比赛前一周（A 期）、比赛后立即（B 期）和比赛后一周（C 期）采集运动员的尿液和血液样本。对尿液样本进行代谢组学分析。检测血液样本中的尿液常规项目和生化指标。
代谢组学结果表明，受试者尿液中包括乳酸、乙酰乙酸和琥珀酸在内的 11 种代谢物的含量在 B 期增加，而二甲胺、胆碱和葡萄糖等 4 种代谢物的含量降低。C 期的大多数代谢物基本恢复到 A 期的水平。上述过程主要涉及丙酮酸代谢、泛酸和 CoA 生物合成、酮体的合成和降解。尿液常规分析结果表明，B 期尿液 pH 值显著下降，PRO 和 URO 水平显著升高，A、C 期三个指标值相似。其他指标在三个阶段之间没有明显差异。血液生化指标分析表明，B 期 LDH、BUN、CK 和 AST 水平显著升高，A、C 期之间无明显差异。这些结果有助于教练合理安排运动员的饮食，并对运动员进行科学训练。
