Wang Lei-Lei, Chen An-Ping, Li Jian-Ying, Sun Zhuo, Yan Shi-Liang, Xu Kai-Yuan
College of Physicial Education, Shanxi University, Taiyuan 030006, China.
Department of health and Natural Sciences, Gdansk University of Physical Education and Sport, 80-336 Gdańsk, Poland.
Healthcare (Basel). 2021 Apr 1;9(4):381. doi: 10.3390/healthcare9040381.
To study the changes in urine metabolism in female water polo players before and after high-intensity training by using ultra-high performance liquid chromatography-mass spectrometry, and to explore the biometabolic characteristics of urine after training and competition.
Twelve young female water polo players (except goalkeepers) from Shanxi Province were selected. A 4-week formal training was started after 1 week of acclimatization according to experimental requirements. Urine samples (5 mL) were collected before formal training, early morning after 4 weeks of training, and immediately after 4 weeks of training matches, and labeled as T1, T2, and T3, respectively. The samples were tested by LC-MS after pre-treatment. XCMS, SIMCA-P 14.1, and SPSS16.0 were used to process the data and identify differential metabolites.
On comparing the immediate post-competition period with the pre-training period (T3 vs. T1), 24 differential metabolites involved in 16 metabolic pathways were identified, among which niacin and niacinamide metabolism and purine metabolism were potential post-competition urinary metabolic pathways in the untrained state of the athletes. On comparing the immediate post-competition period with the post-training period (T3 vs. T2), 10 metabolites involved in three metabolic pathways were identified, among which niacin and niacinamide metabolism was a potential target urinary metabolic pathway for the athletes after training. Niacinamide, 1-methylnicotinamide, 2-pyridone, L-Gln, AMP, and Hx were involved in two metabolic pathways before and after the training.
Differential changes in urine after water polo games are due to changes in the metabolic pathways of niacin and niacinamide.
采用超高效液相色谱 - 质谱联用技术研究女子水球运动员高强度训练前后尿液代谢变化，探讨训练及比赛后尿液的生物代谢特征。
选取山西省12名青年女子水球运动员（不包括守门员）。按照实验要求，在1周适应期后开始为期4周的正式训练。分别在正式训练前、训练4周后的清晨以及训练4周后的比赛结束后立即采集尿液样本（5 mL），依次标记为T1、T2和T3。样本经预处理后采用液相色谱 - 质谱联用仪进行检测。使用XCMS、SIMCA - P 14.1和SPSS16.0软件对数据进行处理并鉴定差异代谢物。
将比赛结束后即刻与训练前时期进行比较（T3 vs. T1），共鉴定出涉及16条代谢途径的24种差异代谢物，其中烟酸和烟酰胺代谢以及嘌呤代谢是运动员在未训练状态下比赛后潜在的尿液代谢途径。将比赛结束后即刻与训练后时期进行比较（T3 vs. T2），鉴定出涉及3条代谢途径的10种代谢物，其中烟酸和烟酰胺代谢是运动员训练后潜在的尿液代谢目标途径。烟酰胺、1 - 甲基烟酰胺、2 - 吡啶酮、L - 谷氨酰胺、AMP和次黄嘌呤在训练前后涉及两条代谢途径。
水球比赛后尿液的差异变化归因于烟酸和烟酰胺代谢途径的改变。
