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在赛沛Xpert Xpress新型冠状病毒检测中未出现N2基因扩增及潜在的新型致病突变：来自新西兰奥克兰的病例系列报道

Lack of N2-gene amplification on the Cepheid Xpert Xpress SARS-CoV-2 assay and potential novel causative mutations: A case series from Auckland, New Zealand.

作者信息

Fox-Lewis Shivani, Fox-Lewis Andrew, Harrower Jay, Chen Richard, Wang Jing, de Ligt Joep, McAuliffe Gary, Taylor Susan, Smit Erasmus

机构信息

Microbiology Department, LabPLUS, Auckland City Hospital, Auckland District Health Board, Auckland, New Zealand.

Microbiology Department, Middlemore Hospital, Auckland District Health Board, Auckland, New Zealand.

出版信息

IDCases. 2021;25:e01233. doi: 10.1016/j.idcr.2021.e01233. Epub 2021 Jul 24.

DOI:10.1016/j.idcr.2021.e01233
PMID:34336597
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8302484/
Abstract

We describe three cases with viral strains that demonstrate impaired N2-gene detection on the Cepheid Xpert Xpress SARS-CoV-2 assay, with two previously undescribed single nucleotide polymorphisms (SNPs): C29197T and G29227T. We propose that these SNPs are likely responsible since they are in close proximity to the previously described C29200T/C29200A SNPs, already shown to abolish N2-gene detection by the Xpert assay. Whether these SNPs abolish N2-gene detection by the Xpert assay individually or only in combination requires more work to elucidate.

摘要

我们描述了三例病毒株的情况，这些病毒株在赛沛Xpert Xpress SARS-CoV-2检测中显示N2基因检测受损，带有两个此前未描述的单核苷酸多态性（SNP）：C29197T和G29227T。我们认为这些SNP可能是原因所在，因为它们与先前描述的C29200T/C29200A SNP非常接近，而后者已被证明会导致Xpert检测无法检测到N2基因。这些SNP是单独导致Xpert检测无法检测到N2基因，还是仅在组合时才会如此，还需要更多研究来阐明。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1c3/8346635/7f6f23cb8a7c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1c3/8346635/7f6f23cb8a7c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1c3/8346635/7f6f23cb8a7c/gr1.jpg

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2
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Emerg Infect Dis. 2021 Mar;27(3):687-693. doi: 10.3201/eid2703.204714. Epub 2021 Jan 5.
3
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Curr Issues Mol Biol. 2023 May 7;45(5):4124-4134. doi: 10.3390/cimb45050262.
4
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J Virol Methods. 2023 Apr;314:114692. doi: 10.1016/j.jviromet.2023.114692. Epub 2023 Feb 15.
5
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6
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7
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Lancet Infect Dis. 2020 Sep;20(9):e238-e244. doi: 10.1016/S1473-3099(20)30484-9. Epub 2020 Jul 3.
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