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长链非编码 RNA PVT1/miR186/KLF5 轴对胆管癌发生发展的影响。

Effect of lncRNA PVT1/miR186/KLF5 Axis on the Occurrence and Progression of Cholangiocarcinoma.

机构信息

General Surgery Department, Zhongshan Hospital, Sun Yat-Sen University, Zhongshan, China.

Organ Transplant Center, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.

出版信息

Biomed Res Int. 2021 Jul 10;2021:8893652. doi: 10.1155/2021/8893652. eCollection 2021.

DOI:10.1155/2021/8893652
PMID:34337058
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8286192/
Abstract

This study primarily focused on the effect of the long noncoding RNA (lncRNA) PVT1/miR186/KLF5 axis on the occurrence and progression of cholangiocarcinoma (CCA). miR186 was found both in the lncRNA PVT1 targeting miRNAs and KLF5 targeting miRNAs using bioinformatic analysis. The expression of lncRNA PVT1 and KLF5 in the TFK-1, QBC939, and HuCCT1 cell lines and normal biliary epithelial HIBEpiC cells was detected by RT-qPCR. The significance of lncRNA PVT1 and KLF5 on cell proliferation was analyzed using the MTT assay and clone formation assay in lncRNA PVT1 and KLF5 silencing HuCCT1 cell lines and lncRNA PVT1and KLF5 overexpressing TFK-1 and QBC939 cell lines, respectively. The potential role of lncRNA PVT1 and KLF5 in cell migration was detected using the transwell invasion assay in CCA cell lines and tumor formation assay. Additionally, lncRNA PVT1 and KLF5 were proved to be highly expressed in CCA tissues and cell lines. Silencing and overexpressing of lncRNA PVT1 or KLF5 markedly inhibited or increased the cell proliferation and cell invasion in CCA cell lines, respectively. Silencing and overexpressing of lncRNA PVT1 significantly inhibited and increased the expression of KLF5 in CCA cell lines, respectively. Silencing of lncRNA PVT1 increased the expression of miR186, and silencing of miR186 increased the expression of KLF5 in CCA cell lines. Cotransfection of lncRNA PVT1 and miR186 increased the expression of KLF5 compared with controls. Overall, these results demonstrated that the lncRNA PVT1/miR186/KLF5 axis might exert a key role in the occurrence and progression of CCA, and this axis might provide a new target for treating CCA.

摘要

本研究主要关注长链非编码 RNA（lncRNA）PVT1/miR186/KLF5 轴对胆管癌（CCA）发生和进展的影响。通过生物信息学分析，在 lncRNA PVT1 靶向 miRNA 和 KLF5 靶向 miRNA 中均发现了 miR186。通过 RT-qPCR 检测 TFK-1、QBC939 和 HuCCT1 细胞系和正常胆管上皮 HIBEpiC 细胞中 lncRNA PVT1 和 KLF5 的表达。在 lncRNA PVT1 沉默 HuCCT1 细胞系和 lncRNA PVT1 和 KLF5 过表达 TFK-1 和 QBC939 细胞系中，通过 MTT 测定和克隆形成测定分析 lncRNA PVT1 和 KLF5 对细胞增殖的意义，分别。通过 CCA 细胞系和肿瘤形成测定中转染小室侵袭测定检测 lncRNA PVT1 和 KLF5 在细胞迁移中的潜在作用。此外，在 CCA 组织和细胞系中证实 lncRNA PVT1 和 KLF5 高度表达。沉默和过表达 lncRNA PVT1 或 KLF5 分别显著抑制和增加 CCA 细胞系中的细胞增殖和细胞侵袭。沉默和过表达 lncRNA PVT1 分别显著抑制和增加 CCA 细胞系中 KLF5 的表达。沉默 lncRNA PVT1 增加了 CCA 细胞系中 miR186 的表达，而沉默 miR186 增加了 KLF5 的表达。与对照相比，共转染 lncRNA PVT1 和 miR186 增加了 KLF5 的表达。总体而言，这些结果表明 lncRNA PVT1/miR186/KLF5 轴可能在 CCA 的发生和发展中发挥关键作用，该轴可能为治疗 CCA 提供新的靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da8a/8286192/0d90effd2566/BMRI2021-8893652.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da8a/8286192/2aaf5b48943c/BMRI2021-8893652.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da8a/8286192/94ebb29ceff6/BMRI2021-8893652.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da8a/8286192/c14afa6f6051/BMRI2021-8893652.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da8a/8286192/a52275dff892/BMRI2021-8893652.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da8a/8286192/7f6bff769def/BMRI2021-8893652.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da8a/8286192/8888712e53fb/BMRI2021-8893652.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da8a/8286192/0d90effd2566/BMRI2021-8893652.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da8a/8286192/2aaf5b48943c/BMRI2021-8893652.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da8a/8286192/94ebb29ceff6/BMRI2021-8893652.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da8a/8286192/c14afa6f6051/BMRI2021-8893652.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da8a/8286192/a52275dff892/BMRI2021-8893652.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da8a/8286192/7f6bff769def/BMRI2021-8893652.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da8a/8286192/8888712e53fb/BMRI2021-8893652.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da8a/8286192/0d90effd2566/BMRI2021-8893652.007.jpg

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