[Generation and characteristics of hybridomas producing monoclonal antibodies to the surface antigen of hepatitis B virus].

An optimal schedule for immunization of BALB/c mice has been found, providing a high yield of hybridoma clones producing monoclonal antibody (MCA) to hepatitis B virus surface antigen (HBsAg). Fourteen hybridomas of ZHAK series have been prepared. The cells of 9 hybridomas secrete MCA to the common-type determinant a, and of 5 hybridomas, to the subtype determinant y of HBsAg. The capacity of hybridoma cells for clone production and for induction of ascitic tumors in syngeneic animals was studied. In the cells of two clones marker chromosomes were detected not occurring in the original parent cells.