Kushch A A, Momot A M, Shumaĭ E P, Bukrinskaia A G, Anan'ev V A
Vopr Virusol. 1987 Jul-Aug;32(4):448-51.
An optimal schedule for immunization of BALB/c mice has been found, providing a high yield of hybridoma clones producing monoclonal antibody (MCA) to hepatitis B virus surface antigen (HBsAg). Fourteen hybridomas of ZHAK series have been prepared. The cells of 9 hybridomas secrete MCA to the common-type determinant a, and of 5 hybridomas, to the subtype determinant y of HBsAg. The capacity of hybridoma cells for clone production and for induction of ascitic tumors in syngeneic animals was studied. In the cells of two clones marker chromosomes were detected not occurring in the original parent cells.
已找到BALB/c小鼠免疫的最佳方案，可高产产生针对乙型肝炎病毒表面抗原（HBsAg）的单克隆抗体（MCA）的杂交瘤克隆。已制备了14个ZHAK系列杂交瘤。9个杂交瘤的细胞分泌针对共同型决定簇a的MCA，5个杂交瘤的细胞分泌针对HBsAg亚型决定簇y的MCA。研究了杂交瘤细胞产生克隆以及在同基因动物中诱导腹水瘤的能力。在两个克隆的细胞中检测到原始亲本细胞中未出现的标记染色体。
