College of Life Sciences, Shihezi University, Shihezi, Xinjiang 832003, China.
Analyst. 2021 Sep 7;146(17):5362-5368. doi: 10.1039/d1an01075a. Epub 2021 Aug 2.
To prevent the transmission of brucellosis, rapid vertical flow technology (RVFT) was developed to detect brucellosis antibodies. To improve the sensitivity of the technique, lipopolysaccharides (LPS) were purified and used to detect brucellosis antibodies. To improve the sensitivity of serum antibody detection, a single multifunctional buffer was established in whole blood and other biological samples, and the advantages of the lateral flow immunoassay were retained. Flower-like gold nanoparticles were applied to RVFT for the first time. In this study, silver ions were catalyzed by flower-like gold nanoparticles into metal silver deposited on the surface of gold nanoparticles for the first time, which not only increased the particle size of gold nanoparticles, but also showed a more distinguishable black color on the test zone, further improving the sensitivity of RVFT. Standard Brucella-positive serum (containing Brucella antibody at 4000 IU mL) could be detected in this system even for a dilution factor of 2 × 10. The detection limit was 2 IU mL. RVFT can effectively avoid the false negative phenomenon in lateral flow immunoassay. RVFT is simple to operate, with a short reaction time, 2-3 minutes visible to the naked eye, without any equipment. Because it is very important to control the brucellosis epidemic, this approach has great application prospects in basic medical units and for veterinarians.
为了预防布氏杆菌病的传播，快速垂直流技术 (RVFT) 被开发出来用于检测布氏杆菌病抗体。为了提高该技术的灵敏度，纯化了脂多糖 (LPS) 并用于检测布氏杆菌病抗体。为了提高血清抗体检测的灵敏度，在全血和其他生物样本中建立了单一多功能缓冲液，并保留了侧流免疫分析的优势。首次将花状金纳米颗粒应用于 RVFT。在这项研究中，银离子首次被花状金纳米颗粒催化成金属银沉积在金纳米颗粒的表面上，这不仅增加了金纳米颗粒的粒径，而且在测试区显示出更明显的黑色，进一步提高了 RVFT 的灵敏度。即使在 2×10 的稀释倍数下，该系统也可以检测到标准的布鲁氏菌阳性血清（含有 4000 IU mL 的布鲁氏菌抗体）。检测限为 2 IU mL。RVFT 可以有效地避免侧流免疫分析中的假阴性现象。RVFT 操作简单，反应时间短，2-3 分钟肉眼可见，无需任何设备。由于控制布氏杆菌病的流行非常重要，因此该方法在基层医疗机构和兽医中具有广阔的应用前景。
