College of Life Sciences, Shihezi University, Xinjiang, Shihezi, P.R. China.
College of Animal Science and Technology, Shihezi University, Xinjiang, Shihezi, P.R. China.
J Appl Microbiol. 2020 Mar;128(3):794-802. doi: 10.1111/jam.14519. Epub 2019 Nov 27.
To prevent the spread of brucellosis, a simple and rapid vertical flow technology (RVFT) for the detection of antibodies targeting brucellosis was developed.
In this study, Brucella sp. lipopolysaccharide was purified and used to detect brucellosis antibodies. Sheep IgG was used as a negative control. Colloidal gold-labeled recombinant staphylococcus aureus protein A was sprayed on a fibreglass membrane to prepare immunogold pads. Rapid vertical flow technology was used to detect Brucella in 1668 Sheep, 2743 bovine, 674 red deer and 420 human samples. The results indicated that the accuracy of this assay can reach 98%.
The established RVFT uses a single multifunctional buffer that can be used to detect antibodies in serum, plasma, whole blood and other biological samples while preserving the advantages of lateral-flow immunoassays.
This technology would be of great use in primary medical units and veterinary stations, and it is of great significance for the control of epidemic diseases.
为了防止布鲁氏菌病的传播，开发了一种简单快速的垂直流动技术（RVFT），用于检测针对布鲁氏菌病的抗体。
在这项研究中，纯化了布鲁氏菌属脂多糖并用其检测布鲁氏菌病抗体。绵羊 IgG 被用作阴性对照。将胶体金标记的重组金黄色葡萄球菌蛋白 A 喷涂在玻璃纤维膜上，制备免疫金垫。使用快速垂直流动技术检测了 1668 只绵羊、2743 头牛、674 只红鹿和 420 个人类样本中的布鲁氏菌。结果表明，该检测方法的准确率可达 98%。
建立的 RVFT 使用单一的多功能缓冲液，可以检测血清、血浆、全血和其他生物样本中的抗体，同时保留了侧向流动免疫分析的优势。
该技术将在基层医疗单位和兽医站得到广泛应用，对传染病的控制具有重要意义。
