College of Life Sciences, Shihezi University, Shihezi 832003, China.
Biosensors (Basel). 2022 Jul 15;12(7):531. doi: 10.3390/bios12070531.
Currently, brucellosis seriously threatens the health of humans and animals and hinders the development of animal husbandry. However, the diagnostic methods for brucellosis have some disadvantages, such as low sensitivity, long detection time, professional operation, and high cost. This study aims to establish a convenient, fast, effective, and inexpensive detection method for brucellosis. Gold nanoparticles with β-cyclodextrin as a reducing agent were prepared and optimized, applied to rapid vertical flow technology (RVFT), and used to establish a kit for the detection of brucellosis. In this study, gold nanoparticles prepared from β-cyclodextrin were applied to RVFT for the first time, and on this basis, silver staining amplification technology was introduced, which further improved the sensitivity and reduced the detection limit of this method. Standard Brucella-Positive Serum (containing Brucella antibody at 4000 IU/mL) could be detected in this system even for a dilution factor of 1 × 10. The detection limit was 4 IU/mL. RVFT is simple to operate, has a short reaction time, and is 5-6 min visible to the naked eye, without any equipment.
目前，布氏杆菌病严重威胁着人类和动物的健康，阻碍了畜牧业的发展。然而，布氏杆菌病的诊断方法存在一些缺点，如灵敏度低、检测时间长、操作专业性强、成本高。本研究旨在建立一种方便、快速、有效、廉价的布氏杆菌病检测方法。本研究以β-环糊精为还原剂制备并优化了金纳米粒子，将其应用于快速垂直流技术（RVFT），并用于建立布氏杆菌病检测试剂盒。本研究首次将β-环糊精制备的金纳米粒子应用于 RVFT，并在此基础上引入银染扩增技术，进一步提高了该方法的灵敏度，降低了检测限。该体系甚至可以检测稀释度为 1×10 的标准布氏杆菌阳性血清（含 4000 IU/mL 的布氏杆菌抗体）。检测限为 4 IU/mL。RVFT 操作简单，反应时间短，肉眼可见，无需任何设备，反应时间为 5-6 分钟。
