College of Food Science and Engineering, Ocean University of China, Qingdao, China.
Qingdao Central Hospital, Qingdao, China.
Neurogastroenterol Motil. 2021 Dec;33(12):e14196. doi: 10.1111/nmo.14196. Epub 2021 Aug 1.
Constipation is a gastrointestinal symptom with high incidence rate and large number of patients. It is becoming one of the urgent medical problems. Poor intestinal motility is one of the important causes of constipation. Current drug treatments for constipation are associated with many side effects; thus, it is necessary to study more effective treatment methods and potential mechanism.
A zebrafish model of intestinal motility obstruction was established by loperamide hydrochloride to evaluate the effect of probiotic, food ingredients, and combination on intestinal peristalsis according to intestinal peristalsis frequency counts. The gastrointestinal survival ability of the best probiotics was evaluated by surface hydrophobicity, self-aggregation, acid and bile salt tolerance, and gastrointestinal transit tolerance. Interactions between probiotics and food ingredients were studied in vivo and in vitro. The expression of 5-HT was detected by ELISA and fluorescence immunoassay, and 5-HT related genes were detected by RT-PCR.
We obtained the probiotics, food ingredients, and combination that effectively promoted intestinal peristalsis, X11 and YRL577, P. persica and KGM, KGM + X11, respectively. Both KGM and P. persica promoted colonization of probiotics in vivo. KGM + X11 could effectively promote the increase in 5-HT synthesis in zebrafish via up-regulating gene expression of TPH-1, TPH-2, and 5-HT and down-regulating gene expression of SERT. The specific in-depth mechanism needs further study.
The combinations of KGM with X11 effectively promoted intestinal peristalsis. We provide a theoretical basis for new modalities that can promote intestinal peristalsis and alleviate constipation.
便秘是一种发病率高、患者数量多的胃肠道症状，已成为亟待解决的医学问题之一。肠道蠕动能力差是引起便秘的重要原因之一。目前便秘的药物治疗方法存在较多副作用，因此，有必要研究更有效的治疗方法和潜在机制。
采用盐酸洛哌丁胺建立斑马鱼肠道蠕动受阻模型，通过计算肠道蠕动频率来评价益生菌、食品成分及其组合对肠道蠕动的影响。采用表面疏水性、自聚集性、耐酸耐胆盐和胃肠道转运能力评价最佳益生菌的胃肠道生存能力。通过体内和体外实验研究益生菌与食品成分的相互作用。通过 ELISA 和荧光免疫测定法检测 5-HT 的表达，通过 RT-PCR 检测 5-HT 相关基因。
我们获得了有效促进肠道蠕动的益生菌、食品成分及其组合，X11 和 YRL577、桃和 KGM、KGM+X11。KGM 和桃均能促进益生菌在体内的定植。KGM+X11 能通过上调 TPH-1、TPH-2 和 5-HT 的基因表达，下调 SERT 的基因表达，有效促进斑马鱼 5-HT 合成的增加。具体的深入机制需要进一步研究。
KGM 与 X11 的组合能有效促进肠道蠕动。为能促进肠道蠕动、缓解便秘的新方法提供了理论依据。
