Cao Jiayuan, Bu Yushan, Hao Haining, Liu Qiqi, Wang Ting, Liu Yisuo, Yi Huaxi
College of Food Science and Engineering, Ocean University of China, Qingdao, China.
Front Nutr. 2022 Jul 6;9:954545. doi: 10.3389/fnut.2022.954545. eCollection 2022.
Hyperuricemia (HUA) is a disorder of purine metabolism resulting in abnormally elevated serum uric acid (UA) concentration. It is believed that there is an association between gut microbiota and HUA, and probiotics have the potential palliative effect. However, the underlying mechanism of probiotics in ameliorating HUA remains unclear. The purpose of this study was to investigate the effect and mechanism of Q7 on HUA in Balb/c mice. The results showed that Q7 had an excellent capability to affect UA metabolism, which could degrade nucleotides by 99.97%, nucleosides by 99.15%, purine by 87.35%, and UA by 81.30%. It was observed that Q7 could downregulate serum UA, blood urea nitrogen (BUN), creatinine (Cr), and xanthine oxidase (XOD) by 47.24%, 14.59%, 54.59%, and 40.80%, respectively. Oral administration of Q7 could restore the liver, kidney, and intestinal injury induced by HUA and the expression of metabolic enzymes and transporters to normal level. 16S rRNA sequencing analysis showed that Q7 treatment could restore the imbalance of species diversity, richness, and community evenness compared with the model group. The ratio of to was recovered nearly to the normal level by Q7 intervention. The dominant microorganisms of Q7 group contained more anti-inflammatory bacteria than those of the model group. These findings indicated that Q7 might regulate UA metabolism and repair the liver and kidney injury by reshaping the gut microbiota and could be used as a potential probiotic strain to ameliorate HUA.
高尿酸血症（HUA）是一种嘌呤代谢紊乱疾病，会导致血清尿酸（UA）浓度异常升高。人们认为肠道微生物群与HUA之间存在关联，并且益生菌具有潜在的缓解作用。然而，益生菌改善HUA的潜在机制仍不清楚。本研究的目的是探讨Q7对Balb/c小鼠高尿酸血症的影响及机制。结果表明，Q7具有出色的影响尿酸代谢的能力，它可以将核苷酸降解99.97%，核苷降解99.15%，嘌呤降解87.35%，尿酸降解81.30%。观察到Q7可使血清尿酸、血尿素氮（BUN）、肌酐（Cr）和黄嘌呤氧化酶（XOD）分别下调47.24%、14.59%、54.59%和40.80%。口服Q7可以使高尿酸血症诱导的肝脏、肾脏和肠道损伤以及代谢酶和转运蛋白的表达恢复到正常水平。16S rRNA测序分析表明，与模型组相比，Q7处理可恢复物种多样性、丰富度和群落均匀度的失衡。通过Q7干预，与的比例几乎恢复到正常水平。Q7组的优势微生物比模型组含有更多的抗炎细菌。这些发现表明，Q7可能通过重塑肠道微生物群来调节尿酸代谢并修复肝肾损伤，并且可以用作改善高尿酸血症的潜在益生菌菌株。
