Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil.
Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Niterói 24210-130, Brazil.
Molecules. 2023 Jun 19;28(12):4843. doi: 10.3390/molecules28124843.
Staphylococci are one of the most common causes of biofilm-related infections. Such infections are hard to treat with conventional antimicrobials, which often lead to bacterial resistance, thus being associated with higher mortality rates while imposing a heavy economic burden on the healthcare system. Investigating antibiofilm strategies is an area of interest in the fight against biofilm-associated infections. Previously, a cell-free supernatant from marine-sponge-associated sp. inhibited staphylococcal biofilm formation and dissociated the mature biofilm. This study aimed to identify the chemical components responsible for the antibiofilm activity of sp. Scanning electron microscopy confirmed that the aqueous extract at the concentration of 32 μg/mL could dissociate the mature biofilm. Liquid chromatography coupled with high-resolution mass spectrometry revealed seven potential compounds in the aqueous extract, including alkaloids, macrolides, steroids, and triterpenes. This study also suggests a possible mode of action on staphylococcal biofilms and supports the potential of sponge-derived as a source of antibiofilm compounds.
葡萄球菌是生物膜相关感染的最常见原因之一。这种感染很难用传统的抗菌药物治疗，因为传统的抗菌药物往往会导致细菌耐药性，从而导致更高的死亡率，并给医疗系统带来沉重的经济负担。研究抗生物膜策略是对抗生物膜相关感染的一个关注领域。此前，从海洋海绵相关的 sp. 中分离出的无细胞上清液抑制了葡萄球菌生物膜的形成并使成熟的生物膜解离。本研究旨在鉴定出 sp. 的抗生物膜活性的化学组成部分。扫描电子显微镜证实，浓度为 32 μg/mL 的水提物可以使成熟的生物膜解离。液相色谱-高分辨质谱联用技术揭示了水提物中的七种潜在化合物，包括生物碱、大环内酯类、甾体和三萜类。本研究还提出了一种抗葡萄球菌生物膜的可能作用模式，并支持海绵衍生的 sp. 作为抗生物膜化合物的潜在来源。
