Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, 517 502, Andhra Pradesh, India.
Department of Veterinary Clinical Complex, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, 517 502, Andhra Pradesh, India.
Trop Anim Health Prod. 2021 Aug 2;53(4):424. doi: 10.1007/s11250-021-02872-3.
Lumpy skin disease (LSD) is an emerging transboundary disease in India. In the recent past, Andhra Pradesh is experiencing outbreaks of LSD in several pockets with a severe economic impact on the farming community. The affected animals showed nodular lesions all over the body in severely affected cases, whereas the lesions were confined to the face, neck, jowl, back, udder, and scrotum in less affected cases. Young ones are highly susceptible to the disease than adults. The mortality was found to be more in young ones than adult cattle which might be due to subsequent secondary bacterial complications. Clinical samples like blood, serum, and tissues were collected randomly from affected animals from four different pockets of Andhra Pradesh. The tissue samples gave positive amplification in PCR targeting LSDV fusion protein gene (ORF 117) and yielded 472 bp product. Another gene specifically targeting ORF036 of LSDV also gave amplification in tissue samples with a product size of 606 bp. The representative samples from four different regions were sequenced for ORF 117 and 036 genes. The phylogeny of the sequenced products of ORF 117 showed more similarity with Kenya Neethling 2490 strain and Russian isolates of 2019. In addition, the phylogeny of ORF 036 showed the path of entry of the virus into the country and also to Andhra Pradesh. The isolates showed similarity with the isolates of India (Odisha), Bangladesh, Russia, Egypt, and Kenya. These studies paved way for the future perspective of developing a vaccine to control the disease.
块状皮肤病（LSD）是印度一种新出现的跨境疾病。在最近的过去，安得拉邦的几个地区都爆发了 LSD，给农业社区带来了严重的经济影响。受影响的动物在严重感染的情况下全身出现结节性病变，而在较轻感染的情况下病变仅限于面部、颈部、颌部、背部、乳房和阴囊。幼畜比成年畜更容易感染该病。幼畜的死亡率高于成年牛，这可能是由于随后的继发细菌性并发症。从安得拉邦的四个不同地区的受感染动物身上随机采集了血液、血清和组织等临床样本。针对 LSDV 融合蛋白基因（ORF117）的 PCR 靶向组织样本，得到阳性扩增，产生 472bp 的产物。另一个针对 LSDV 的 ORF036 的特定基因在组织样本中也得到了扩增，产物大小为 606bp。对来自四个不同地区的代表性样本进行 ORF117 和 ORF036 基因测序。ORF117 测序产物的系统发育树显示与肯尼亚 Neethling 2490 株和俄罗斯 2019 年分离株的相似度更高。此外，ORF036 的系统发育显示了病毒进入该国并进入安得拉邦的途径。这些分离株与印度（奥里萨邦）、孟加拉国、俄罗斯、埃及和肯尼亚的分离株具有相似性。这些研究为未来开发疫苗控制该疾病铺平了道路。
