Central Disease Investigation Laboratory (CDIL), 48, KaziAlauddin Road, Dhaka, People's Republic of Bangladesh.
Department of Livestock Services, Dhaka, People's Republic of Bangladesh.
BMC Vet Res. 2021 Jan 29;17(1):61. doi: 10.1186/s12917-021-02751-x.
Lumpy skin disease (LSD) is a contagious viral disease of cattle caused by lumpy skin disease virus (LSDV). LSD has recently spread in Asia following outbreaks in the Middle East and Europe. The disease emerged in Bangladesh in July 2019 in the Chattogram district, then rapidly spread throughout the entire country. We investigated six LSD outbreaks in Bangladesh to record the clinical signs and collect samples for diagnostic confirmation. Furthermore, we performed the molecular characterization of Bangladesh isolates, analyzing the full RPO30 and GPCR genes and the partial EEV glycoprotein gene.
Clinical observations revealed common LSD clinical signs in the affected cattle. PCR and real-time PCR, showed the presence of the LSDV genome in samples from all six districts. Phylogenetic analysis and detailed inspection of multiple sequence alignments revealed that Bangladesh isolates differ from common LSDV field isolates encountered in Africa, the Middle East, and Europe, as well as newly emerged LSDV variants in Russia and China. Instead, they were closely related to LSDV KSGP-0240, LSDV NI2490, and LSDV Kenya.
These results show the importance of continuous monitoring and characterization of circulating strains and the need to continually refine the strategies for differentiating vaccine strains from field viruses.
块状皮肤病（LSD）是一种由块状皮肤病病毒（LSDV）引起的牛传染性病毒性疾病。LSD 最近在中东和欧洲爆发后在亚洲传播。该疾病于 2019 年 7 月在孟加拉国的 Chattogram 区首次出现，随后迅速蔓延至全国。我们调查了孟加拉国的六次 LSD 暴发事件，记录了临床症状并收集了用于诊断确认的样本。此外，我们对孟加拉国分离株进行了分子特征分析，分析了完整的 RPO30 和 GPCR 基因以及 EEV 糖蛋白基因的部分序列。
临床观察显示，受影响的牛群中存在常见的 LSD 临床症状。PCR 和实时 PCR 显示，来自六个地区的样本均存在 LSDV 基因组。系统发育分析和对多个序列比对的详细检查表明，孟加拉国分离株与在非洲、中东和欧洲发现的常见 LSDV 田间分离株以及俄罗斯和中国新出现的 LSDV 变体不同，而是与 LSDV KSGP-0240、LSDV NI2490 和 LSDV Kenya 密切相关。
这些结果表明，持续监测和表征循环株的重要性，以及不断完善区分疫苗株和田间病毒的策略的必要性。
