Fürst P, Krüger C, Meemken H A, Groebel W
Chemisches Landesuntersuchungsamt Nordrhein-Westfalen, Münster, Bundesrepublik Deutschland.
Z Lebensm Unters Forsch. 1987 Nov;185(5):394-7. doi: 10.1007/BF01042261.
Tetrachlorobenzyltoluenes, which represent the major constituent of the PCB substitutes Ugilec 141 and Ugilec T, have been widely used in the last few years as hydraulic fluids in underground mining. Their use has been termed a "closed application", but there are obviously considerable losses. Tetrachlorobenzyltoluenes released underground can enter the environment vit pia water, mine outputs or ventilation systems and as a consequence they can contaminate the food-chain, especially in fish. In fish from areas where there is extensive mining concentrations of tetrachlorobenzyltoluenes in edible portions ranged up to 25 mg/kg. The compounds were extracted from the freeze-dried material with petroleum ether. Gel chromatography on Bio-Beads S-X3 was chosen for the removal of fat followed by adsorption chromatography on silica gel according to the method of Specht and Tillkes. A combination of gas chromatography/mass spectrometry employing an ion trap detector was used for the separation and detection of the compounds. The great selectivity of mass selective detection combined with the high resolving power of capillary gas chromatography made it possible to determine and confirm the presence of Ugilec in fish down to a level of 0.02 mg/kg, even if there was a large PCB surplus.
四氯苄基甲苯是多氯联苯替代品Ugilec 141和Ugilec T的主要成分，在过去几年中被广泛用作地下采矿中的液压油。它们的使用被称为“封闭应用”，但显然存在相当大的损失。地下释放的四氯苄基甲苯可通过水、矿井排水或通风系统进入环境，因此它们会污染食物链，尤其是鱼类。在广泛采矿地区的鱼类中，可食用部分的四氯苄基甲苯浓度高达25毫克/千克。这些化合物从冻干材料中用石油醚提取。根据Specht和Tillkes的方法，选择在Bio-Beads S-X3上进行凝胶色谱法去除脂肪，然后在硅胶上进行吸附色谱法。采用离子阱检测器的气相色谱/质谱联用技术用于化合物的分离和检测。质量选择性检测的高选择性与毛细管气相色谱的高分辨率相结合，即使存在大量多氯联苯过剩，也能够测定并确认鱼类中Ugilec的存在，检测下限可达0.02毫克/千克。
