Drug Delivery, Disposition and Dynamics and ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Monash Institute of Pharmaceutical Sciences, Monash University (Parkville Campus), 381 Royal Parade, Parkville, VIC, 3052, Australia.
Australian Synchrotron, ANSTO, 800 Blackburn Rd, Clayton, VIC, 3168, Australia.
Colloids Surf B Biointerfaces. 2021 Nov;207:112005. doi: 10.1016/j.colsurfb.2021.112005. Epub 2021 Jul 26.
Lipid-based liquid crystalline (LLC) systems are formed by the self-assembly of lipid materials in aqueous environments. The internal nanostructures of LLC systems can be manipulated using remote stimuli and have the potential to serve as 'on-demand' drug delivery systems. In this study, a magnetically-responsive system that displayed a transition in nanostructure from liposomes to cubosomes/hexasomes under external alternating magnetic field (AMF) was established by the incorporation of iron oxide nanoparticles (IONPs) into a PEGylated phytantriol (PHYT)-based LLC system. Small angle X-ray scattering (SAXS) was utilized to assess the equilibrium phase behaviour of the systems with different compositions of the lipids to find the optimized formulation. Time-resolved SAXS was then used to determine the dynamic transformation of nanostructures of the IONP-containing systems with the activation of AMF. The formulation containing PHYT and DSPE-PEG2000 at a 95 to 5 molar percent ratio produced a transition from lamellar phase to bicontinuous cubic phase, showing a slow-to-fast drug release profile. Inclusion of either 5 nm or 15 nm IONPs imparted magnetic-responsiveness to the system. The magnetically-responsive system produced an 'on-demand' drug delivery system from which the drug release was able to be triggered externally by AMF-stimulation.
基于脂质的液晶（LLC）体系是由脂质材料在水相环境中自组装形成的。可以使用远程刺激来操纵 LLC 体系的内部纳米结构，并且有可能作为“按需”药物递送系统。在这项研究中，通过将氧化铁纳米粒子（IONP）掺入聚乙二醇化植物三醇（PHYT）基 LLC 体系中，建立了一种在外加交变磁场（AMF）下从脂质体向立方/六方囊泡转变的磁响应体系。小角 X 射线散射（SAXS）用于评估不同脂质组成的系统的平衡相行为，以找到最佳配方。然后，使用时间分辨 SAXS 来确定含有 AMF 激活的 IONP 的系统的纳米结构的动态转变。含有 PHYT 和 DSPE-PEG2000 的配方摩尔比为 95 比 5 时，从层状相转变为双连续立方相，表现出缓慢到快速的药物释放曲线。包含 5nm 或 15nm IONP 可赋予体系磁响应性。该磁响应体系产生了一种“按需”药物递送系统，通过 AMF 刺激可以从外部触发药物释放。
