Laboratory of Toxicology and Medicinal Plants, Anhanguera-Uniderp University, Campo Grande, MS, Brazil.
Postgraduate Program in Infectious and Parasitic Diseases, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil.
Photochem Photobiol Sci. 2021 Aug;20(8):1069-1085. doi: 10.1007/s43630-021-00081-3. Epub 2021 Aug 2.
The purpose of this study was to assess the topic use of Sebastiania hispida extract and low-level gallium-arsenide laser irradiation (GaAs, 904 nm) to reduce the local myonecrosis and edema of Bothrops moojeni snake venom-injected gastrocnemius. Wistar rats receiving intramuscular venom injection (VBm) were compared with saline control (S) and envenomed rats receiving local exposure to plant extract (VExt) or laser irradiation (VL). The phytochemistry and thin-layer chromatography of S. hispida extract indicated the presence of phenolic compounds like gallic acid and flavonoids including quercetin. Gastrocnemius of VExt and VL groups had a significant reduction of edema and creatine kinase (CK) activities and a greater Myogenin (MyoG) expression compared to VBm group, with the plant extract efficacy better than laser exposure. Reduction of edema and serum CK activities reflects a lessening of muscle damage, whereas the increase of MyoG indicates myoblast differentiation and acceleration of muscle repair. The S. hispida richness in phenolic compounds and flavonoids, such as the light modulatory ability to triggering a multitude of cell signalings likely underlie the positive outcomes. Our findings suggest both treatments as potential auxiliary tools to be explored in clinical trials in combination with anti-venom therapy after Bothropic snakebites.
本研究旨在评估 Sebastiania hispida 提取物和低水平砷化镓激光照射（GaAs，904nm）对减少巴西矛头蝮蛇毒液注射引起的比目鱼肌局部坏死和水肿的作用。接受肌肉内毒液注射（VBm）的 Wistar 大鼠与盐水对照（S）和接受植物提取物（VExt）或激光照射（VL）局部暴露的中毒大鼠进行比较。S. hispida 提取物的植物化学和薄层色谱分析表明存在酚类化合物，如没食子酸和类黄酮，包括槲皮素。与 VBm 组相比，VExt 和 VL 组的水肿和肌酸激酶（CK）活性明显降低，Myogenin（MyoG）表达增加，植物提取物的疗效优于激光照射。水肿和血清 CK 活性的降低反映了肌肉损伤的减轻，而 MyoG 的增加表明成肌细胞分化和肌肉修复加速。S. hispida 富含酚类化合物和类黄酮，如光调节能力，可能触发多种细胞信号转导，这可能是其积极作用的基础。我们的研究结果表明，这两种治疗方法都可能作为辅助工具，与抗蛇毒疗法联合应用于巴西矛头蝮蛇咬伤后的临床试验中。
