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天然分解枯木的宏基因组、宏转录组和微生物组。

Metagenomes, metatranscriptomes and microbiomes of naturally decomposing deadwood.

机构信息

Laboratory of Environmental Microbiology, Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 14220, Praha 4, Czech Republic.

Department of Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ, Permoserstraße 15, 04318, Leipzig, Germany.

出版信息

Sci Data. 2021 Aug 3;8(1):198. doi: 10.1038/s41597-021-00987-8.

Abstract

Deadwood represents significant carbon (C) stock in a temperate forests. Its decomposition and C mobilization is accomplished by decomposer microorganisms - fungi and bacteria - who also supply the foodweb of commensalist microbes. Due to the ecosystem-level importance of deadwood habitat as a C and nutrient stock with significant nitrogen fixation, the deadwood microbiome composition and function are critical to understanding the microbial processes related to its decomposition. We present a comprehensive suite of data packages obtained through environmental DNA and RNA sequencing from natural deadwood. Data provide a complex picture of the composition and function of microbiome on decomposing trunks of European beech (Fagus sylvatica L.) in a natural forest. Packages include deadwood metagenomes, metatranscriptomes, sequences of total RNA, bacterial genomes resolved from metagenomic data and the 16S rRNA gene and ITS2 metabarcoding markers to characterize the bacterial and fungal communities. This project will be of use to microbiologists, environmental biologists and biogeochemists interested in the microbial processes associated with the transformation of recalcitrant plant biomass.

摘要

枯木代表了温带森林中重要的碳(C)储量。其分解和 C 释放是由分解微生物——真菌和细菌——完成的,它们也为共生微生物的食物网提供了养分。由于枯木生境作为 C 和养分储备的生态系统水平重要性,具有显著固氮作用,因此枯木微生物组的组成和功能对于理解与其分解相关的微生物过程至关重要。我们提供了一整套通过环境 DNA 和 RNA 测序从天然枯木中获得的数据包。这些数据提供了一个复杂的图景,展示了在天然森林中,正在分解的欧洲山毛榉(Fagus sylvatica L.)树干上微生物组的组成和功能。这些数据包包括枯木宏基因组、宏转录组、总 RNA 序列、从宏基因组数据中解析出的细菌基因组以及 16S rRNA 基因和 ITS2 代谢组学标记,以表征细菌和真菌群落。该项目将对微生物学家、环境生物学家和生物地球化学家有用,他们对与难分解植物生物质转化相关的微生物过程感兴趣。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4920/8333335/b0c50d1a96f7/41597_2021_987_Fig1_HTML.jpg

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