转录组测序揭示了与狮头鹅成肌细胞增殖和分化相关的信号通路。
Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts.
作者信息
Chen Jiahui, Zhang Shuai, Chen Genghua, Deng Xianqi, Zhang Danlu, Wen Huaqiang, Yin Yunqian, Lin Zetong, Zhang Xiquan, Luo Wen
机构信息
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affair, South China Agricultural University, Guangzhou 510642, China.
出版信息
Animals (Basel). 2022 Oct 27;12(21):2956. doi: 10.3390/ani12212956.
Chinese Shitou goose is a type of large goose with high meat yield. Understanding the genetic regulation of muscle development in Shitou goose would be beneficial to improve the meat production traits of geese. Muscle development is regulated by genes related to myoblast proliferation and differentiation. In this study, the RNA-seq method was used to construct the mRNA and lncRNA expression profiles of Shitou goose myoblasts and myotubes. A total of 1664 differentially expressed (DE) mRNAs and 244 DE-lncRNAs were identified. The alternative mRNA splicing in proliferation and differentiation stages was also analyzed. Notably, pathways enriched in DE-mRNAs, DE-splicing transcripts, and DE-lncRNAs all point to the Wnt signaling pathway, indicating that the Wnt signaling is a key regulatory pathway of muscle development in Shitou goose. We also constructed the interactive network of DE-lncRNAs and DE-mRNAs and revealed some key genes of lncRNAs regulating the proliferation and differentiation of myoblasts. These results provide new insights for the study of the muscle development of the Shitou goose.
中国狮头鹅是一种产肉量高的大型鹅。了解狮头鹅肌肉发育的遗传调控将有助于提高鹅的产肉性状。肌肉发育受与成肌细胞增殖和分化相关的基因调控。在本研究中，采用RNA测序方法构建了狮头鹅成肌细胞和肌管的mRNA和lncRNA表达谱。共鉴定出1664个差异表达的mRNA和244个差异表达的lncRNA。还分析了增殖和分化阶段的可变mRNA剪接。值得注意的是，差异表达的mRNA、差异剪接转录本和差异表达的lncRNA富集的通路均指向Wnt信号通路，表明Wnt信号是狮头鹅肌肉发育的关键调控通路。我们还构建了差异表达的lncRNA和差异表达的mRNA的相互作用网络，并揭示了一些调控成肌细胞增殖和分化的lncRNA关键基因。这些结果为狮头鹅肌肉发育的研究提供了新的见解。
Zotero 插件