Huang Rongqin, Chen Jiahui, Dong Xu, Zhang Xiquan, Luo Wen
College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, China.
Animals (Basel). 2024 Feb 8;14(4):576. doi: 10.3390/ani14040576.
CircRNA, a recently characterized non-coding RNA (ncRNA) variant, functions as a molecular sponge, exerting regulatory control by binding to microRNA (miRNA) and modulating the expression of downstream proteins, either promoting or inhibiting their expression. Among poultry species, geese hold significant importance, prized by consumers for their delectable taste and rich nutritional content. Despite the prominence of geese, research on the growth and development of goose muscle, particularly the regulatory role of circRNAs in goose muscle formation, remains insufficiently explored. In this study, we constructed comprehensive expression profiles of circRNAs and messenger RNAs (mRNAs) within the myoblasts and myotubes of Shitou geese. We identified a total of 96 differentially expressed circRNAs (DEcircRNAs) and 880 differentially expressed mRNAs (DEmRNAs). Notably, the parental genes of DEcircRNAs and DEmRNAs exhibited enrichment in the Wnt signaling pathway, highlighting its potential impact on the proliferation and differentiation of goose myoblasts. Employing RNAhybrid and miRDB, we identified circRNA-miRNA pairs and mRNA-miRNA pairs that may play a role in regulating myogenic differentiation or muscle growth. Subsequently, utilizing Cytoscape, we constructed a circRNA-miRNA-mRNA interaction network aimed at unraveling the intricate regulatory mechanisms involved in goose muscle growth and development, which comprises 93 circRNAs, 351 miRNAs, and 305 mRNAs. Moreover, the identification of 10 hub genes (, , , , , , , , , and ) potentially linked to myogenesis, along with the exploration of their circRNA-miRNA-hub gene regulatory axis, was also conducted. These competitive endogenous RNA (ceRNA) regulatory networks elucidate the molecular regulatory mechanisms associated with muscle growth in Shitou geese, providing deeper insights into the reciprocal regulation of circRNA, miRNA, and mRNA in the context of goose muscle formation.
环状RNA（circRNA）是一种最近被鉴定出来的非编码RNA（ncRNA）变体，它作为一种分子海绵，通过与微小RNA（miRNA）结合并调节下游蛋白质的表达来发挥调控作用，既可以促进也可以抑制其表达。在家禽物种中，鹅具有重要意义，因其美味的口感和丰富的营养成分而受到消费者的青睐。尽管鹅很重要，但关于鹅肌肉生长发育的研究，特别是circRNA在鹅肌肉形成中的调控作用，仍未得到充分探索。在本研究中，我们构建了狮头鹅成肌细胞和肌管内circRNA和信使RNA（mRNA）的综合表达谱。我们共鉴定出96个差异表达的circRNA（DEcircRNA）和880个差异表达的mRNA（DEmRNA）。值得注意的是，DEcircRNA和DEmRNA的亲本基因在Wnt信号通路中富集，突出了其对鹅成肌细胞增殖和分化的潜在影响。利用RNAhybrid和miRDB，我们鉴定了可能在调节肌源性分化或肌肉生长中起作用的circRNA-miRNA对和mRNA-miRNA对。随后，利用Cytoscape，我们构建了一个circRNA-miRNA-mRNA相互作用网络，旨在揭示参与鹅肌肉生长发育的复杂调控机制，该网络包含93个circRNA、351个miRNA和305个mRNA。此外，还鉴定了10个可能与肌生成相关的枢纽基因（、、、、、、、、和），并探索了它们的circRNA-miRNA-枢纽基因调控轴。这些竞争性内源RNA（ceRNA）调控网络阐明了与狮头鹅肌肉生长相关的分子调控机制，为circRNA、miRNA和mRNA在鹅肌肉形成过程中的相互调控提供了更深入的见解。
