He Qi, Song Xianmei, Ma Hongwen, Yin Yanbo
Institution of Crop Research, Ningxia Academy of Agricultural and Forestry Science, Yinchuan, People's Republic of China.
School of Agriculture, Ningxia University, Yinchuan, People's Republic of China.
Mitochondrial DNA B Resour. 2021 Jul 15;6(8):2363-2365. doi: 10.1080/23802359.2021.1951138. eCollection 2021.
(Motschulsky) (Coleoptera: Chrysomelidae) is an important agricultural insect pest. In this study, the complete mitochondrial genome of was sequenced using Illumina HiSeq X Ten. The mitogenome was 16,213 bp long and comprised 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs) and a putative control region (CR). The nucleotide composition of the mitochondrial genome was significantly biased (A, G, C and T accounted for 41.04%, 8.01%, 11.76% and 39.18%, respectively) with 80.23% A + T content. Two rRNAs were located between tRNA-Leu and the CR, separated by tRNA-Val. The CR, located between 12 s rRNA and tRNA-Ile, was 1661 bp long. The length of the 22 tRNAs ranged from 61 to 71 bp. Phylogenetic analyses of 29 Chrysomelidae-Galerucinae species based on 13 mitochondrial protein-coding genes reconstructed using Bayesian 3.2.0 showed that the mitogenome was clustered with the existing three different species of the genus mitogenomes in a monophyletic manner. The mitogenome provides an important data resource for further studies and contributes to our understanding of the phylogeny of this group.
(莫氏叶甲)(鞘翅目:叶甲科)是一种重要的农业害虫。在本研究中,使用Illumina HiSeq X Ten对(莫氏叶甲)的完整线粒体基因组进行了测序。该线粒体基因组长度为16213 bp,包含13个蛋白质编码基因(PCGs)、两个核糖体RNA基因(rRNAs)、22个转移RNA基因(tRNAs)和一个假定的控制区(CR)。(莫氏叶甲)线粒体基因组的核苷酸组成存在显著偏差(A、G、C和T分别占41.04%、8.01%、11.76%和39.18%),A+T含量为80.23%。两个rRNAs位于tRNA - Leu和CR之间,被tRNA - Val隔开。CR位于12 s rRNA和tRNA - Ile之间,长度为1661 bp。22个tRNAs的长度范围为61至71 bp。基于使用贝叶斯3.2.0重建的13个线粒体蛋白质编码基因对29种叶甲科 - 龟甲亚科物种进行的系统发育分析表明,(莫氏叶甲)的线粒体基因组与(莫氏叶甲属)现有的三种不同物种的线粒体基因组以单系方式聚类。(莫氏叶甲)的线粒体基因组为进一步研究提供了重要的数据资源,并有助于我们对该类群系统发育的理解。