Departamento de Bioquímica, Biología Molecular y Celular, Universidad de Zaragoza, Zaragoza, Spain.
Unidad de Reproducción Asistida, Hospital Universitario Príncipe de Asturias, Universidad Complutense de Madrid, Alcalá de Henares, Madrid, Spain.
J Assist Reprod Genet. 2023 Mar;40(3):671-682. doi: 10.1007/s10815-023-02721-2. Epub 2023 Jan 26.
To analyze the influence of Caucasian mitochondrial haplogroups on controlled ovarian stimulation outcome (COS), embryo (E), and pregnancy success.
In a Caucasian population (n = 517) undergoing COS, mitochondrial haplogroups and physiological parameters were determined. Patients were classified, according to Bologna criteria, as good (>3)/poor ≤3) responder, on dependence of recruited oocytes (RO), and in pregnancy/non-pregnancy groups. Haplogroups were determined by sequencing mitochondrial hypervariable sequence I and confirmed by polymerase chain reaction (PCR), followed by restriction fragment length polymorphisms (RFLP).
The rank of total dose of FSH (TD FSH) was similar in all clusters/haplogroups, except in JT, which is narrower (950-3,650 IU), particularly in T (1,350-3,650 IU). The statistical analysis showed higher RO and E in JT when compared to U, although it was only Uk which accumulated significantly in pregnancy respect to JT. Pearson's correlations between TD FSH and RO showed negative statistical significance in all population (P = 0.001), H (P = 0.03), JT (P = 0.01), and T (P = 0.03). The percentage of contribution of TD FSH on RO was almost nine times in the JT cluster as compared to all population one.
JT cluster shows a different influence of TD FSH on RO. JT cluster shows higher RO and E than U, but it is Uk which exhibits a significant higher pregnancy rate than JT. The negative influence of the JT cluster on pregnancy success strongly suggests that the m.4216 T > C polymorphism could be responsible.
分析高加索人线粒体单倍群对控制性卵巢刺激（COS）结局、胚胎（E）和妊娠成功的影响。
在接受 COS 的高加索人群（n=517）中，确定了线粒体单倍群和生理参数。根据博洛尼亚标准，根据募集卵母细胞（RO）将患者分为好（>3）/差（≤3）反应者，并分为妊娠/非妊娠组。通过测序线粒体高变区 I 确定单倍群，并通过聚合酶链反应（PCR）确认，随后进行限制性片段长度多态性（RFLP）分析。
除 JT 外，所有聚类/单倍群的总促卵泡激素剂量（TD FSH）的等级相似，JT 更窄（950-3650IU），尤其是 T（1350-3650IU）。统计分析显示，与 U 相比，JT 中的 RO 和 E 更高，但只有 Uk 在妊娠方面与 JT 相比有显著积累。在所有人群（P=0.001）、H（P=0.03）、JT（P=0.01）和 T（P=0.03）中，TD FSH 与 RO 之间的 Pearson 相关性均显示出统计学意义上的负相关。在 JT 聚类中，TD FSH 对 RO 的贡献百分比几乎是所有人群的九倍。
JT 聚类显示出 TD FSH 对 RO 的不同影响。JT 聚类显示出比 U 更高的 RO 和 E，但 Uk 的妊娠率明显高于 JT。JT 聚类对妊娠成功的负面影响强烈表明 m.4216 T>C 多态性可能是其原因。
