Zhu Ting, Yao Jia, Liu Hao, Zhou Chen-Hao, Liu Yuan-Zhan, Wang Zheng-Wei, Quan Zhe-Xue, Li Bo, Yang Ji, Huang Wei-Chang, Nie Ming
Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, National Observations and Research Station for Wetland Ecosystems of the Yangtze Estuary, Institute of Biodiversity Science and Institute of Eco-Chongming, School of Life Sciences, Fudan University, Shanghai, 200438, China.
Eastern China Conservation Centre for Wild Endangered Plant Resources, Shanghai Chenshan Botanical Garden, Shanghai, 201602, China.
Environ Microbiol. 2021 Oct;23(10):6210-6222. doi: 10.1111/1462-2920.15703. Epub 2021 Aug 11.
The foliar surface forms one of the largest aboveground habitats on Earth and maintains plant-fungus relationships that greatly affect ecosystem functioning. Despite many studies with particular plant species, the foliar epiphytic mycobiome has not been studied across a large number of plant species from different taxa. Using high-throughput sequencing, we assessed epiphytic mycobiomes on leaf surfaces of 592 plant species in a botanical garden. Plants of angiosperms, gymnosperms, and pteridophytes were involved. Plant taxonomy, leaf side, growing environment, and evolutionary relationships were considered. We found that pteridophytes showed the higher fungal species diversity, stronger mutualistic fungal interactions, and a greater percentage of putative pathogens than gymnosperms and angiosperms. Plant taxonomic group, leaf side, and growing environment were significantly associated with the foliar epiphytic mycobiome, but the similarity of the mycobiomes among plants was not directly related to the distance of the host evolutionary tree. Our results provide a general understanding of the foliar fungal mycobiomes from pteridophytes to angiosperms. These findings will facilitate our understanding of foliar fungal epiphytes and their roles in plant communities and ecosystems.
叶表面构成了地球上最大的地上栖息地之一，并维持着对生态系统功能有重大影响的植物 - 真菌关系。尽管对特定植物物种进行了许多研究，但尚未对来自不同分类群的大量植物物种的叶附生真菌群落进行研究。我们使用高通量测序技术，评估了一个植物园中592种植物叶表面的附生真菌群落。涉及被子植物、裸子植物和蕨类植物。考虑了植物分类学、叶的正反面、生长环境和进化关系。我们发现，与裸子植物和被子植物相比，蕨类植物表现出更高的真菌物种多样性、更强的互利真菌相互作用以及更高比例的推定病原体。植物分类群、叶的正反面和生长环境与叶附生真菌群落显著相关，但植物间真菌群落的相似性与宿主进化树的距离没有直接关系。我们的结果提供了对从蕨类植物到被子植物的叶真菌群落的总体认识。这些发现将有助于我们理解叶附生真菌及其在植物群落和生态系统中的作用。
