Zhu Chunchao, Lin Yumiao, Wang Zihui, Luo Wenqi, Zhang Yonghua, Chu Chengjin
Department of Bioengineering, Zhuhai Campus of Zunyi Medical University, Zhuhai, China.
Goldpac Limited, Zhuhai, China.
Front Microbiol. 2023 Mar 17;14:1147285. doi: 10.3389/fmicb.2023.1147285. eCollection 2023.
Microorganisms can influence plant growth and health, ecosystem functioning, and stability. Community and network structures of mangrove phyllosphere fungi have rarely been studied although mangroves have very important ecological and economical values. Here, we used high throughput sequencing of the internal transcribed spacer 2 (ITS2) to assess epiphytic and endophytic phyllosphere fungal communities of six true mangrove species and five mangrove associates. Totally, we obtained 1,391 fungal operational taxonomic units (OTUs), including 596 specific epiphytic fungi, 600 specific endophytic fungi, and 195 shared fungi. The richness and community composition differed significantly for epiphytes and endophytes. Phylogeny of the host plant had a significant constraint on epiphytes but not endophytes. Network analyses showed that plant-epiphyte and plant-endophyte networks exhibited strong specialization and modularity but low connectance and anti-nestedness. Compared to plant-endophyte network, plant-epiphyte network showed stronger specialization, modularity, and robustness but lower connectance and anti-nestedness. These differences in community and network structures of epiphytes and endophytes may be caused by spatial niche partitioning, indicating their underlying ecological and environmental drivers are inconsistent. We highlight the important role of plant phylogeny in the assembly of epiphytic but not endophytic fungal communities in mangrove ecosystems.
微生物能够影响植物生长与健康、生态系统功能及稳定性。尽管红树林具有非常重要的生态和经济价值，但红树林叶际真菌的群落和网络结构却鲜有研究。在此，我们利用内转录间隔区2（ITS2）的高通量测序技术，评估了六种真红树植物和五种半红树植物的附生和内生叶际真菌群落。我们总共获得了1391个真菌操作分类单元（OTU），包括596种特异性附生真菌、600种特异性内生真菌和195种共享真菌。附生真菌和内生真菌的丰富度及群落组成存在显著差异。宿主植物的系统发育对附生真菌有显著限制，但对内生真菌没有。网络分析表明，植物 - 附生真菌和植物 - 内生真菌网络表现出很强的特异性和模块性，但连接性和非嵌套性较低。与植物 - 内生真菌网络相比，植物 - 附生真菌网络表现出更强的特异性、模块性和稳健性，但连接性和非嵌套性较低。附生真菌和内生真菌在群落和网络结构上的这些差异可能是由空间生态位划分引起的，这表明它们潜在的生态和环境驱动因素并不一致。我们强调了植物系统发育在红树林生态系统中附生而非内生真菌群落组装中的重要作用。
