• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

KDM1A 调控组蛋白去乙酰化抑制 DACT1 表达对宫颈癌增殖和迁移的影响。

Histone Deacetylation Regulated by KDM1A to Suppress DACT1 in Proliferation and Migration of Cervical Cancer.

机构信息

No. 3 Obstetrics and Gynecology Department, Hunan Provincial People's Hospital, No. 61, West Jiefang Road, Furong District, Changsha, Hunan 410005, China.

出版信息

Anal Cell Pathol (Amst). 2021 Jul 24;2021:5555452. doi: 10.1155/2021/5555452. eCollection 2021.

DOI:10.1155/2021/5555452
PMID:34350095
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8328692/
Abstract

OBJECTIVE

Increased expression of KDM1A and decreased expression of DACT1 in cervical cancer cells were noticed in a previous study. This study is aimed at exploring the mechanism behind the KDM1A regulation on DACT1 in cervical cancer cells.

METHODS

The expression profile of KDM1A and DACT1 in cervical cancer tissues was searched in TCGA database. In vitro experiments verified the effect of KDM1A and DACT1 on proliferation and migration ability of cervical cancer cell lines after cell transfection. The interaction of KDM1A with HDAC1 was identified by coimmunoprecipitation (Co-IP). The expression levels of KDM1A and DACT1 in cervical cancer cell lines were determined by qRT-PCR and western blot.

RESULTS

TCGA database showed that cervical cancer tissues had elevated expression of KDM1A and decreased expression of DACT1, which was consistent with the observation in cervical cancer cell lines. KDM1A was found to negatively regulate DACT1 through histone deacetylation. Meanwhile, the downregulation of KDM1A or overexpression of DACT1 could suppress the cell proliferation and migration ability in HeLa and SiHa cells. Cotransfection of KDM1A and DACT1 overexpression could reverse the increased cell proliferation and migration ability induced by KDM1A overexpression.

CONCLUSION

KDM1A can downregulate DACT1 expression through histone deacetylation and therefore suppress the proliferation and migration of cervical cancer cells.

摘要

目的

在之前的研究中注意到宫颈癌细胞中 KDM1A 的表达增加和 DACT1 的表达减少。本研究旨在探讨 KDM1A 对宫颈癌细胞中 DACT1 的调节机制。

方法

在 TCGA 数据库中搜索宫颈癌组织中 KDM1A 和 DACT1 的表达谱。通过细胞转染，体外实验验证了 KDM1A 和 DACT1 对宫颈癌细胞系增殖和迁移能力的影响。通过共免疫沉淀（Co-IP）鉴定 KDM1A 与 HDAC1 的相互作用。通过 qRT-PCR 和 Western blot 确定宫颈癌细胞系中 KDM1A 和 DACT1 的表达水平。

结果

TCGA 数据库显示，宫颈癌组织中 KDM1A 的表达升高，DACT1 的表达降低，与宫颈癌细胞系中的观察结果一致。KDM1A 通过组蛋白去乙酰化负调控 DACT1。同时，下调 KDM1A 或过表达 DACT1 可抑制 HeLa 和 SiHa 细胞的增殖和迁移能力。共转染 KDM1A 和 DACT1 过表达可逆转 KDM1A 过表达诱导的细胞增殖和迁移能力增加。

结论

KDM1A 可以通过组蛋白去乙酰化下调 DACT1 的表达，从而抑制宫颈癌细胞的增殖和迁移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c941/8328692/795add554ee9/ACP2021-5555452.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c941/8328692/95f0bc3c471a/ACP2021-5555452.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c941/8328692/e25f9970c304/ACP2021-5555452.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c941/8328692/877e10267e79/ACP2021-5555452.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c941/8328692/1db39584f9ed/ACP2021-5555452.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c941/8328692/795add554ee9/ACP2021-5555452.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c941/8328692/95f0bc3c471a/ACP2021-5555452.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c941/8328692/e25f9970c304/ACP2021-5555452.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c941/8328692/877e10267e79/ACP2021-5555452.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c941/8328692/1db39584f9ed/ACP2021-5555452.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c941/8328692/795add554ee9/ACP2021-5555452.005.jpg

相似文献

1
Histone Deacetylation Regulated by KDM1A to Suppress DACT1 in Proliferation and Migration of Cervical Cancer.KDM1A 调控组蛋白去乙酰化抑制 DACT1 表达对宫颈癌增殖和迁移的影响。
Anal Cell Pathol (Amst). 2021 Jul 24;2021:5555452. doi: 10.1155/2021/5555452. eCollection 2021.
2
Oncogene LSD1 is epigenetically suppressed by miR-137 overexpression in human non-small cell lung cancer.在人类非小细胞肺癌中，原癌基因LSD1通过miR-137的过表达在表观遗传上受到抑制。
Biochimie. 2017 Jun;137:12-19. doi: 10.1016/j.biochi.2017.02.010. Epub 2017 Feb 20.
3
Oncogenic function of DACT1 in colon cancer through the regulation of β-catenin.DACT1 在结肠癌中的致癌功能是通过调节 β-连环蛋白实现的。
PLoS One. 2012;7(3):e34004. doi: 10.1371/journal.pone.0034004. Epub 2012 Mar 21.
4
[Bcl-2 associated athanogene 3 affects the epithelial-mesenchymal transition in human cervical cancer].[Bcl-2相关生原基因3影响人宫颈癌中的上皮-间质转化]
Zhonghua Fu Chan Ke Za Zhi. 2017 Aug 25;52(8):551-557. doi: 10.3760/cma.j.issn.0529-567X.2017.08.009.
5
MicroRNA-106b is involved in transforming growth factor β1-induced cell migration by targeting disabled homolog 2 in cervical carcinoma.微小RNA-106b通过靶向宫颈癌中的失活同源物2参与转化生长因子β1诱导的细胞迁移。
J Exp Clin Cancer Res. 2016 Jan 15;35:11. doi: 10.1186/s13046-016-0290-6.
6
Knockdown of KDM1A suppresses tumour migration and invasion by epigenetically regulating the TIMP1/MMP9 pathway in papillary thyroid cancer.KDM1A 的敲低通过表观遗传调控 TIMP1/MMP9 通路抑制甲状腺乳头状癌的肿瘤迁移和侵袭。
J Cell Mol Med. 2019 Aug;23(8):4933-4944. doi: 10.1111/jcmm.14311. Epub 2019 Jun 18.
7
Dapper homolog 1 is a novel tumor suppressor in gastric cancer through inhibiting the nuclear factor-κB signaling pathway.Dapper 同源物 1 通过抑制核因子-κB 信号通路在胃癌中作为一种新型的肿瘤抑制因子。
Mol Med. 2012 Dec 20;18(1):1402-11. doi: 10.2119/molmed.2012.00243.
8
CD73 promotes proliferation and migration of human cervical cancer cells independent of its enzyme activity.CD73促进人宫颈癌细胞的增殖和迁移，且不依赖其酶活性。
BMC Cancer. 2017 Feb 15;17(1):135. doi: 10.1186/s12885-017-3128-5.
9
TGF-β-induced DACT1 biomolecular condensates repress Wnt signalling to promote bone metastasis.TGF-β 诱导的 DACT1 生物分子凝聚物抑制 Wnt 信号传导以促进骨转移。
Nat Cell Biol. 2021 Mar;23(3):257-267. doi: 10.1038/s41556-021-00641-w. Epub 2021 Mar 9.
10
MiR-137-3p Inhibits Colorectal Cancer Cell Migration by Regulating a KDM1A-Dependent Epithelial-Mesenchymal Transition.miR-137-3p 通过调控 KDM1A 依赖的上皮-间充质转化抑制结直肠癌细胞迁移。
Dig Dis Sci. 2021 Jul;66(7):2272-2282. doi: 10.1007/s10620-020-06518-6. Epub 2020 Aug 4.

引用本文的文献

1
FTO alleviated the diabetic nephropathy progression by regulating the N6-methyladenosine levels of DACT1.FTO通过调节DACT1的N6-甲基腺苷水平减轻糖尿病肾病进展。
Open Life Sci. 2025 May 5;20(1):20221049. doi: 10.1515/biol-2022-1049. eCollection 2025.
2
Alpha-linolenic acid-mediated epigenetic reprogramming of cervical cancer cell lines.α-亚麻酸介导的宫颈癌细胞系表观遗传重编程
Epigenetics. 2025 Dec;20(1):2451551. doi: 10.1080/15592294.2025.2451551. Epub 2025 Feb 2.
3
The Role of DACT Family Members in Tumorigenesis and Tumor Progression.

本文引用的文献

1
Screening and Discovery of New Potential Biomarkers and Small Molecule Drugs for Cervical Cancer: A Bioinformatics Analysis.宫颈癌新型潜在生物标志物和小分子药物的筛选与发现：生物信息学分析。
Technol Cancer Res Treat. 2020 Jan-Dec;19:1533033820980112. doi: 10.1177/1533033820980112.
2
DNA methylation markers as triage test for the early identification of cervical lesions in a Chinese population.DNA 甲基化标志物作为一种甄别试验，用于早期识别中国人群中的宫颈病变。
Int J Cancer. 2021 Apr 1;148(7):1768-1777. doi: 10.1002/ijc.33430. Epub 2020 Dec 20.
3
Biological and clinical aspects of HPV-related cancers.
DACT 家族成员在肿瘤发生和肿瘤进展中的作用。
Int J Biol Sci. 2022 Jul 11;18(11):4532-4544. doi: 10.7150/ijbs.70784. eCollection 2022.
人乳头瘤病毒相关癌症的生物学和临床方面。
Cancer Biol Med. 2020 Nov 15;17(4):864-878. doi: 10.20892/j.issn.2095-3941.2020.0370. Epub 2020 Dec 15.
4
Uptake of cervical cancer screening service and associated factors among age-eligible women in Ethiopia: systematic review and meta-analysis.埃塞俄比亚符合年龄条件的女性宫颈癌筛查服务的接受情况及相关因素：系统评价与荟萃分析
Infect Agent Cancer. 2020 Nov 13;15(1):67. doi: 10.1186/s13027-020-00334-3.
5
A newly identified lncRNA H1FX-AS1 targets DACT1 to inhibit cervical cancer via sponging miR-324-3p.一种新鉴定出的长链非编码RNA H1FX-AS1通过吸附miR-324-3p靶向DACT1来抑制宫颈癌。
Cancer Cell Int. 2020 Jul 31;20:358. doi: 10.1186/s12935-020-01385-7. eCollection 2020.
6
LSD1/KDM1A inhibitors in clinical trials: advances and prospects.在临床试验中的 LSD1/KDM1A 抑制剂：进展与展望。
J Hematol Oncol. 2019 Dec 4;12(1):129. doi: 10.1186/s13045-019-0811-9.
7
Cytoplasmic translocation of nuclear LSD1 () in human hepatoma cells is induced by its inhibitors.核赖氨酸特异性去甲基化酶1（LSD1）在人肝癌细胞中的细胞质易位是由其抑制剂诱导的。
Hepat Oncol. 2019 Jun 5;6(2):HEP13. doi: 10.2217/hep-2018-0008.
8
Effects of DACT1 methylation status on invasion and metastasis of nasopharyngeal carcinoma.DACT1 甲基化状态对鼻咽癌侵袭转移的影响。
Biol Res. 2019 Jun 10;52(1):31. doi: 10.1186/s40659-019-0238-3.
9
The function of histone acetylation in cervical cancer development.组蛋白乙酰化在宫颈癌发展中的作用。
Biosci Rep. 2019 Apr 12;39(4). doi: 10.1042/BSR20190527. Print 2019 Apr 30.
10
Significant association between KDM1A promoter hypomethylation and colorectal cancer in Han Chinese.KDM1A启动子低甲基化与中国汉族人群结直肠癌之间存在显著关联。
Pathol Res Pract. 2019 Mar;215(3):532-538. doi: 10.1016/j.prp.2018.12.005. Epub 2018 Dec 7.