解脲脲原体菌株tetM基因的克隆与鉴定
Cloning and characterization of tetM gene from a Ureaplasma urealyticum strain.
作者信息
Brown J T, Roberts M C
机构信息
Department of Pathobiology, University of Washington, Seattle 98177.
出版信息
Antimicrob Agents Chemother. 1987 Nov;31(11):1852-4. doi: 10.1128/AAC.31.11.1852.
Abstract
We have cloned a 4.9-kilobase (kb) HincII fragment which contains a tetracycline resistance determinant (tetM) from the chromosome of Ureaplasma urealyticum. The 4.9-kb HincII fragment contains DNA in addition to the structural gene, is closely related to the previously characterized 5.0-kb fragment from pJI3, and contains no normal ureaplasmal DNA sequences.
摘要
我们克隆了一个4.9千碱基（kb）的HincII片段，该片段含有来自解脲脲原体染色体的四环素抗性决定簇（tetM）。这个4.9 kb的HincII片段除了结构基因外还包含其他DNA，与之前鉴定的来自pJI3的5.0 kb片段密切相关，并且不包含正常的脲原体DNA序列。
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