Center for Bioscience Research and Education, Utsunomiya University, Tochigi, Japan.
Department of Engineering, Kitami Institute of Technology, Kitami, Japan.
Plant Signal Behav. 2021 Nov 2;16(11):1963104. doi: 10.1080/15592324.2021.1963104. Epub 2021 Aug 5.
CFP/YFP-paired FRET is routinely used to estimate intracellular Ca concentrations . This system, however, is excited with blue light, which is likely to invoke unexpected responses in plant cells. This report describes a new green light-excitable FRET system with an mKO2/mCherry pair. Plant cells expressing this newly constructed FRET system demonstrated its ability to monitor changes in cytosolic free calcium concentration. The new system is likely to find applications in studies of plant cells where undesirable blue light responses must be avoided.
CFP/YFP 荧光共振能量转移（FRET）技术常被用于估计细胞内钙离子浓度。然而，该系统采用蓝光激发，这可能会在植物细胞中引发意想不到的反应。本报告描述了一种新的基于 mKO2/mCherry 对的绿光激发 FRET 系统。表达该新型 FRET 系统的植物细胞展示了其监测细胞质游离钙离子浓度变化的能力。该新系统有望在需要避免不良蓝光反应的植物细胞研究中得到应用。
