Sorbonne Université, CNRS, Laboratoire Jean Perrin (UMR 8237), 4 place Jussieu, F-75005, Paris, France.
Université Côte d'Azur, CNRS UMR 7010, Institut de Physique de Nice, Parc Valrose, 06108, Nice, France.
NPJ Biofilms Microbiomes. 2021 Aug 5;7(1):64. doi: 10.1038/s41522-021-00233-4.
Multispecies microbial adherent communities are widespread in nature and organisms, although the principles of their assembly and development remain unclear. Here, we test the possibility of establishing a simplified but relevant model of multispecies biofilm in a non-invasive laboratory setup for the real-time monitoring of community development. We demonstrate that the four chosen species (Bacillus thuringiensis, Pseudomonas fluorescens, Kocuria varians, and Rhodocyclus sp.) form a dynamic community that deterministically reaches its equilibrium after ~30 h of growth. We reveal the emergence of complexity in this simplified community as reported by an increase in spatial heterogeneity and non-monotonic developmental kinetics. Importantly, we find interspecies interactions consisting of competition for resources-particularly oxygen-and both direct and indirect physical interactions. The simplified experimental model opens new avenues to the study of adherent bacterial communities and their behavior in the context of rapid global change.
多物种微生物附着群落广泛存在于自然界和生物体内，尽管其组装和发展的原则仍不清楚。在这里，我们测试了在非侵入性实验室设置中建立简化但相关的多物种生物膜模型的可能性，以便实时监测群落发展。我们证明，所选的四种物种（苏云金芽孢杆菌、荧光假单胞菌、变栖克雷伯氏菌和红环菌）形成了一个动态群落，在生长约 30 小时后确定性地达到平衡。我们揭示了这种简化群落中出现的复杂性，表现为空间异质性增加和非单调发育动力学。重要的是，我们发现种间相互作用包括对资源（特别是氧气）的竞争，以及直接和间接的物理相互作用。简化的实验模型为研究附着细菌群落及其在快速全球变化背景下的行为开辟了新的途径。
