Patil Shankargouda, Ashi Heba, Hosmani Jagadish, Almalki Abdulrahman Yahya, Alhazmi Yaser Ali, Mushtaq Shazia, Parveen Sameena, Baeshen Hosam Ali, Varadarajan Saranya, Raj A Thirumal, Patil Vikrant R, Vyas Nishant
Department of Maxillofacial Surgery and Diagnostic Sciences, Division of Oral Pathology, College of Dentistry Jazan University, Jazan, Saudi Arabia.
Department of Dental Public Health, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia.
Saudi J Biol Sci. 2021 Aug;28(8):4553-4559. doi: 10.1016/j.sjbs.2021.04.056. Epub 2021 Apr 27.
has been applied successfully as an anti-inflammatory, anti-diabetic, and even as an anti-cancer agent. Yet, to date, the application of Giloy has not been explored concerning oral cancer.
To assess the effect of extract (TcE) on an oral cancer cell line.
AW13516 (oral cancer cell line) cells were treated with the prepared aqueous extract of TcE for 24 h at various concentrations ranging between 5 μg/ml and 100 μg/ml and compared with control (cells without treatment). Thee effect of the extracts on apoptosis was assessed by through Annexin V flow cytometry assay and Luminometry based assessment of Caspase 8, 9 and caspase 3/7 activity. RNA was isolated from treated cells and gene expression of selected metastatic genes (MMP1, MMP10, and CXCL8); epithelial-mesenchymal stem cell genes (TWIST1, SNAIL, ZEB1, Oct4) and stemness related genses (Nanog, Sox2) were analyzed by using a quantitative real-time PCR system. The experiments were performed in triplicates.
Aqueous extract of TcE was found to induce apoptosis inducer in AW13516 cells in a concentration-dependent manner and was potent even at a low concentration of 5 μg/ml. The apoptosis induction was confirmed with the caspase activity assay. Treatment of the cells with the extract for 24 h exhibited a significant decrease in the expression of EMT genes in a dose-dependent manner without an effect on the metastatic genes.
Aqueous extract of TcE induces apoptosis-mediated cell death in the oral cancer cell line AW13516 while attenuating its potential for epithelial mesenchymal transition.
已成功用作抗炎、抗糖尿病甚至抗癌药物。然而，迄今为止，尚未探讨匙羹藤在口腔癌方面的应用。
评估匙羹藤提取物（TcE）对口腔癌细胞系的作用。
将AW13516（口腔癌细胞系）细胞用制备好的不同浓度（5μg/ml至100μg/ml）的TcE水提取物处理24小时，并与对照组（未处理的细胞）进行比较。通过膜联蛋白V流式细胞术检测和基于荧光素酶检测法评估半胱天冬酶8、9和半胱天冬酶3/7的活性，来评估提取物对细胞凋亡的影响。从处理过的细胞中分离RNA，并使用定量实时PCR系统分析选定的转移基因（MMP1、MMP10和CXCL8）、上皮-间充质干细胞基因（TWIST1、SNAIL、ZEB1、Oct4）和干性相关基因（Nanog、Sox2）的基因表达。实验重复进行三次。
发现TcE水提取物以浓度依赖性方式诱导AW13516细胞凋亡，即使在低浓度5μg/ml时也有效。通过半胱天冬酶活性测定证实了细胞凋亡诱导作用。用提取物处理细胞24小时后，EMT基因的表达呈剂量依赖性显著降低，而对转移基因没有影响。
TcE水提取物在口腔癌细胞系AW13516中诱导凋亡介导的细胞死亡，同时减弱其上皮-间充质转化的潜能。
