Hadad Sahar El, Alsolami Maha, Aldahlawi Alia, Alrahimi Jehan, Basingab Fatemah, Hassoubah Shahira, Alothaid Hani
Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
Research Center of Genetic Engineering and Bioinformatics, VACSERA, Cairo, Egypt.
Saudi J Biol Sci. 2021 Aug;28(8):4751-4761. doi: 10.1016/j.sjbs.2021.04.090. Epub 2021 May 6.
Probiotics have attracted considerable attention because of their ability to ameliorate disease and prevent cancer. In this study, we examined the immunomodulatory effects of a probiotic on the intestinal mucosa azoxymethane-induced colon cancer. Sixty female mice were divided into four groups (n = 15 each). One group of untreated mice was used as a control (C group). Another mouse group was injected with azoxymethane once weekly for 8 weeks to induce colon cancer (CC group). Finally, two groups of mice were continuously treated twice per week from week 2 to 16 with either the (Lac CC group) or (Strep CC group) bacterial strain pre-and post-treatment as performed for the CC group. Remarkably, , , , , , and transcription were significantly downregulated in the Strep CC intestinal mucosa group. Additionally, IL2 expression was decreased significantly in the Strep CC mouse serum, whereas TNFα was remarkably elevated compared to that in the CC, Lac CC, and untreated groups. This study suggested that did not interrupt or hinder colon cancer development in mice when administered as a prophylactic.
由于益生菌具有改善疾病和预防癌症的能力，它们已引起了相当大的关注。在本研究中，我们检测了一种益生菌对肠道黏膜由氧化偶氮甲烷诱导的结肠癌的免疫调节作用。60只雌性小鼠被分为四组（每组n = 15）。一组未处理的小鼠用作对照（C组）。另一组小鼠每周注射一次氧化偶氮甲烷，持续8周以诱导结肠癌（CC组）。最后，两组小鼠从第2周开始至第16周每周连续处理两次，分别使用 （乳酸CC组）或 （链球菌CC组）菌株，处理前后情况与CC组相同。值得注意的是，链球菌CC肠道黏膜组中 、 、 、 、 及 转录显著下调。此外，链球菌CC小鼠血清中白细胞介素2表达显著降低，而与CC组、乳酸CC组和未处理组相比，肿瘤坏死因子α显著升高。本研究表明，作为预防性给药时， 不会干扰或阻碍小鼠结肠癌的发展。
