Pojer Jonathan M, Manning Samuel A, Kroeger Benjamin, Kondo Shu, Harvey Kieran F
Peter MacCallum Cancer Centre, 305 Grattan St, Melbourne, VIC 3000, Australia.
Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, VIC 3010, Australia.
iScience. 2021 Jul 9;24(8):102830. doi: 10.1016/j.isci.2021.102830. eCollection 2021 Aug 20.
The Hippo pathway is a conserved signaling network that regulates organ growth and cell fate. One such cell fate decision is that of R8 photoreceptor cells in the eye, where Hippo specifies whether cells sense blue or green light. We show that only a subset of proteins that control organ growth via the Hippo pathway also regulate R8 cell fate choice, including the STRIPAK complex, Tao, Pez, and 14-3-3 proteins. Furthermore, key Hippo pathway proteins were primarily cytoplasmic in R8 cells rather than localized to specific membrane domains, as in cells of growing epithelial organs. Additionally, Warts was the only Hippo pathway protein to be differentially expressed between R8 subtypes, while central Hippo pathway proteins were expressed at dramatically lower levels in adult and pupal eyes than in growing larval eyes. Therefore, we reveal several important differences in Hippo signaling in the contexts of organ growth and cell fate.
Hippo信号通路是一个保守的信号网络，可调节器官生长和细胞命运。眼睛中R8光感受器细胞的细胞命运决定就是其中之一，在这种情况下，Hippo信号通路决定细胞是感知蓝光还是绿光。我们发现，只有一部分通过Hippo信号通路控制器官生长的蛋白质也调节R8细胞命运的选择，包括STRIPAK复合体、Tao、Pez和14-3-3蛋白。此外，关键的Hippo信号通路蛋白在R8细胞中主要位于细胞质中，而不像在生长中的上皮器官细胞那样定位于特定的膜结构域。此外，Warts是唯一在R8亚型之间差异表达的Hippo信号通路蛋白，而核心Hippo信号通路蛋白在成虫和蛹期眼睛中的表达水平比幼虫生长阶段的眼睛中低得多。因此，我们揭示了Hippo信号通路在器官生长和细胞命运方面的几个重要差异。
