Department of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, 12403Huazhong University of Science and Technology, Wuhan, China.
Department of General Surgery, The Central Hospital of Wuhan, Tongji Medical College, 12403Huazhong University of Science and Technology, Wuhan, China.
Technol Cancer Res Treat. 2021 Jan-Dec;20:15330338211033074. doi: 10.1177/15330338211033074.
Previous studies reported that N-myc downstream-regulated gene 1 (NDRG1) was upregulated in various cancer tissues and decreased expression of miR-188-3p and miR-133b could suppress cell proliferation, metastasis, and invasion and induce apoptosis of cancer cells. However, the molecular mechanism of NRDG1 involved in hepatocellular carcinoma (HCC) tumorigenesis is still unknown.
The expressions of miR-188-3p, miR-133b, and NRDG1 in HCC tissues and cells were quantified by qRT-PCR and Western blot. MTT assay and transwell invasion assay were performed to evaluate cell growth and cell migration, respectively. Luciferase reporter assay were performed to determine whether miR-188-3p and miR-133b could directly bind to NRDG1 in HCC cells.
The results showed that NRDG1 was upregulated and these 2 microRNAs were downregulated in HCC tissues. NRDG1 was negatively correlated with miR-188-3p and miR-133b in HCC tissues. MiR-188-3p and miR-133b were demonstrated to directly bind to 3'UTR of NRDG1 and inhibit its expression. Upregulation of miR-188-3p and miR-133b reduced NRDG1 expression in hepatocellular carcinoma cell lines, which consequently inhibited cell growth and cell migration.
Our finding suggested that miR-188-3p and miR-133b exert a suppressive effect on hepatocellular carcinoma proliferation, invasion, and migration through downregulation of NDRG1.
先前的研究表明，N- myc 下游调节基因 1（NDRG1）在各种癌症组织中上调，而 miR-188-3p 和 miR-133b 的表达下调可抑制癌细胞的增殖、转移和侵袭，并诱导癌细胞凋亡。然而，NRDG1 参与肝细胞癌（HCC）肿瘤发生的分子机制尚不清楚。
通过 qRT-PCR 和 Western blot 定量检测 HCC 组织和细胞中 miR-188-3p、miR-133b 和 NRDG1 的表达。MTT 检测和 Transwell 侵袭实验分别用于评估细胞生长和细胞迁移。荧光素酶报告实验用于确定 miR-188-3p 和 miR-133b 是否可以直接结合 HCC 细胞中的 NRDG1。
结果表明，NRDG1 在 HCC 组织中上调，这两种 microRNAs 在 HCC 组织中下调。NRDG1 与 HCC 组织中的 miR-188-3p 和 miR-133b 呈负相关。miR-188-3p 和 miR-133b 被证明可以直接结合 NRDG1 的 3'UTR 并抑制其表达。miR-188-3p 和 miR-133b 的上调降低了肝癌细胞系中 NRDG1 的表达，从而抑制了细胞的生长和迁移。
我们的研究结果表明，miR-188-3p 和 miR-133b 通过下调 NDRG1 对肝细胞癌的增殖、侵袭和迁移发挥抑制作用。
