Abedin-Do Atieh, Zhang Ze, Douville Yvan, Méthot Mireille, Rouabhia Mahmoud
Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Québec, QC G1V 0A6, Canada.
Axe Médecine Régénératrice, Centre de Recherche du CHU de Québec, Département de Chirurgie, Faculté de Médecine, Université Laval, Québec, QC G1V 0A6, Canada.
Biology (Basel). 2021 Jul 9;10(7):641. doi: 10.3390/biology10070641.
Diabetic foot ulcers are indicative of an impaired wound healing process. This delay may be resolved through electrical stimulation (ES). The goal of the present study was to evaluate the effect of ES on diabetic fibroblast adhesion and growth, and the secretion of cytokines and growth factors. Diabetic human skin fibroblasts (DHSF) were exposed to various intensities of direct current ES (100, 80, 40 and 20 mV/mm). The effect of ES on fibroblast adhesion and growth was evaluated using Hoechst staining, MTT and trypan blue exclusion assays. The secretion of cytokine and growth factor was assessed by cytokine array and ELISA assay. The long-term effects of ES on DHSF shape and growth were determined by optical microscopy and cell count. We demonstrated that ES at 20 and 40 mV/mm promoted cell adhesion, viability and growth. ES also decreased the secretion of pro-inflammatory cytokines IL-6 and IL-8 yet promoted growth factor FGF7 secretion during 48 h post-ES. Finally, the beneficial effect of ES on fibroblast growth was maintained up to 5 days post-ES. Overall results suggest the possible use of low-intensity direct current ES to promote wound healing in diabetic patients.
糖尿病足溃疡表明伤口愈合过程受损。这种延迟可能通过电刺激（ES）来解决。本研究的目的是评估电刺激对糖尿病成纤维细胞黏附、生长以及细胞因子和生长因子分泌的影响。将糖尿病患者皮肤成纤维细胞（DHSF）暴露于不同强度的直流电刺激（100、80、40和20 mV/mm）下。使用Hoechst染色、MTT和台盼蓝排斥试验评估电刺激对成纤维细胞黏附及生长的影响。通过细胞因子阵列和酶联免疫吸附测定法评估细胞因子和生长因子的分泌情况。通过光学显微镜和细胞计数确定电刺激对糖尿病患者皮肤成纤维细胞形态和生长的长期影响。我们证明，20和40 mV/mm的电刺激可促进细胞黏附、活力和生长。电刺激还可减少促炎细胞因子IL-6和IL-8的分泌，但在电刺激后48小时内促进生长因子FGF7的分泌。最后，电刺激对成纤维细胞生长的有益作用在电刺激后长达5天内得以维持。总体结果表明，低强度直流电刺激可能有助于促进糖尿病患者的伤口愈合。
