Matey José Manuel, López-Fernández Adrián, García-Ruiz Carmen, Montalvo Gemma, Zapata Félix, Martínez María A
Department of Chemistry and Drugs, National Institute of Toxicology and Forensic Sciences, Calle José Echegaray 4, Las Rozas de Madrid, 28232 Madrid, Spain.
Instituto Universitario de Investigación en Ciencias Policiales (IUICP), Universidad de Alcalá, Calle Libreros 27, Alcalá de Henares, 28801 Madrid, Spain.
Toxics. 2021 Jul 15;9(7):170. doi: 10.3390/toxics9070170.
The analysis of drugs of abuse in hair and other biological matrices of forensic interest requires great selectivity and sensitivity. This has been traditionally achieved through target analysis, using one or more analytical methods that include different preanalytical stages, and more complex procedures followed by toxicological laboratories. There is no exception with 2C-series drugs, such as 2C-B, a new psychoactive substance (NPS), which use has emerged and significantly increased, year by year, in the last decades. Continuously new analytical methods are required to selectively detect and identify these new marketed substances at very low concentrations. In this case report, one former case of a polydrug consumer (charged of a crime against public health in Spain) was reanalyzed in hair matrix. In this reanalysis, 2C-B has been positively detected and identified using liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS/MS). The most selective analytical UHPLC-HRMS/MS method alongside a universal and simpler pretreatment methodology has opened up more possibilities for the detection of substances of different chemical structure and optimization of different HRMS/MS detection approaches allowing the identification of 2-CB in the hair of a real forensic case.
对毛发及其他具有法医学意义的生物基质中的滥用药物进行分析需要极高的选择性和灵敏度。传统上，这是通过靶向分析来实现的，即使用一种或多种分析方法，包括不同的分析前阶段，以及毒理学实验室采用的更复杂程序。2C系列药物，如新型精神活性物质（NPS）2C-B，也不例外。在过去几十年中，其使用量逐年显著增加。需要不断研发新的分析方法，以在极低浓度下选择性地检测和识别这些新上市的物质。在本病例报告中，对一名曾滥用多种药物的使用者（在西班牙被控一项危害公众健康罪）的毛发样本进行了重新分析。在此次重新分析中，使用超高效液相色谱-高分辨率质谱联用仪（UHPLC-HRMS/MS）对2C-B进行了阳性检测和鉴定。最具选择性的UHPLC-HRMS/MS分析方法与通用且更简单的预处理方法相结合，为检测不同化学结构的物质以及优化不同的HRMS/MS检测方法开辟了更多可能性，从而能够在实际法医案件的毛发中鉴定出2-CB。
