Castro Tassia Flavia Dias, Carneiro William Franco, Reichel Tharyn, Fabem Sarah Lacerda, Machado Mônica Rodrigues Ferreira, de Souza Krisnanda Kelly Castro, Resende Luciane Vilela, Murgas Luis David Solis
Faculty of Animal Science and Veterinary Medicine, Department of Veterinary Medicine, Federal University of Lavras, Lavras, MG, CEP:37200-000, Brazil.
School of Agricultural Sciences, Department of Agriculture, Federal University of Lavras, Lavras, MG, Brasil.
Toxicol Res (Camb). 2022 Oct 3;11(5):891-899. doi: 10.1093/toxres/tfac067. eCollection 2022 Oct.
is a herbaceous plant found in tropical and subtropical regions. In vivo pharmacological parameters show that leaf extracts of this plant have antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities due to their bioactive compounds such as flavonoids and phenols. Despite the evidence for several bioactivities of , information on its safety and tolerability is limited. The objective of this study was to assess the effect and concentration of different extracts of on the development of zebrafish () embryos. To study the impact of aqueous (AE), ethanolic (EE), and methanolic (ME) extracts, the embryos were exposed to 0.625, 1.25, 2.5, 5, and 10 mg mL for up to 120-h postfertilization to assess embryonic developmental toxicity and then to 0.039, 0.078, 0.156, 0.312, and 0.625 mg mL to assess the antioxidant responses of the enzymes superoxide dismutase catalase, glutathione S-transferase (GST), and cell apoptosis. The results showed that, depending on the extraction solvent, concentration used, and exposure time, extracts caused mortality, altered the hatching time, and promoted changes in enzymatic activities. Delays in development and increased GST activity were found in all treatments. Apoptosis was not observed in any of the treatments. In conclusion, AE, EE, and ME concentrations above 0.625 mg mL can cause adverse effects on the early stages of zebrafish development.
是一种生长在热带和亚热带地区的草本植物。体内药理学参数表明，由于其黄酮类和酚类等生物活性化合物，该植物的叶提取物具有抗氧化、抗炎、抗糖尿病和抗菌活性。尽管有证据表明具有多种生物活性，但其安全性和耐受性方面的信息有限。本研究的目的是评估不同提取物对斑马鱼胚胎发育的影响和浓度。为了研究水提取物（AE）、乙醇提取物（EE）和甲醇提取物（ME）的影响，将胚胎在受精后长达120小时暴露于0.625、1.25、2.5、5和10mg/mL，以评估胚胎发育毒性，然后暴露于0.039、0.078、0.156、0.312和0.625mg/mL，以评估超氧化物歧化酶、过氧化氢酶、谷胱甘肽S-转移酶（GST）的抗氧化反应和细胞凋亡。结果表明，根据提取溶剂、使用浓度和暴露时间的不同，提取物会导致死亡率升高、孵化时间改变，并促进酶活性变化。在所有处理中均发现发育延迟和GST活性增加。在任何处理中均未观察到细胞凋亡。总之，浓度高于0.625mg/mL的AE、EE和ME会对斑马鱼发育的早期阶段产生不利影响。
