Westfalewicz Błażej, Słowińska Mariola, Judycka Sylwia, Ciereszko Andrzej, Dietrich Mariola A
Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
Animals (Basel). 2021 Jul 5;11(7):2013. doi: 10.3390/ani11072013.
The age of the bull is widely accepted to influence the production of sperm, affecting the amount and quality of produced semen, which in turn impacts the results of cryopreservation. However, the exact influence of the maturation process on cryopreserved sperm, as well as the underlying molecular mechanisms of this process, are not fully understood. The goal of this study was to evaluate changes in the proteome of thawed semen (spermatozoa and supernatant) collected from young and adult bulls ( = 6) using the 2D-DIGE approach. The quality of semen was assessed using a CASA system and flow cytometry. We found no significant age-related variation in semen quality, with the exception of the average path velocity of sperm movement, which was higher in adult bulls. Proteomic analysis indicated 15 spermatozoa proteins and 10 supernatant proteins with significant age-related changes. Our results suggest that semen from adult bulls is better equipped with proteins related to energy production, protection of spermatozoa against oxidative stress and fertilizing ability. Proteins increased in abundance in young bull spermatozoa were connected to the cytoskeleton and its development, which strongly suggests that developmental processes are still in progress. In conclusion, our results provide novel insight into the mechanism of the development of the male reproductive system of cattle.
普遍认为公牛的年龄会影响精子的产生，进而影响精液的产量和质量，而这反过来又会影响冷冻保存的结果。然而，成熟过程对冷冻精子的确切影响以及该过程的潜在分子机制尚未完全明确。本研究的目的是使用二维差异凝胶电泳（2D-DIGE）方法评估从年轻公牛和成年公牛（n = 6）采集的解冻精液（精子和上清液）蛋白质组的变化。使用计算机辅助精子分析（CASA）系统和流式细胞术评估精液质量。我们发现，除成年公牛精子运动的平均路径速度较高外，精液质量没有与年龄相关的显著差异。蛋白质组学分析表明，有15种精子蛋白和10种上清液蛋白存在与年龄相关的显著变化。我们的结果表明，成年公牛的精液含有更多与能量产生、保护精子免受氧化应激和受精能力相关的蛋白质。在年轻公牛精子中丰度增加的蛋白质与细胞骨架及其发育有关，这强烈表明发育过程仍在进行。总之，我们的结果为牛雄性生殖系统的发育机制提供了新的见解。
