Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, Tuwima 10, 10-748 Olsztyn, Poland.
Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, Tuwima 10, 10-748 Olsztyn, Poland.
Anim Reprod Sci. 2020 Oct;221:106552. doi: 10.1016/j.anireprosci.2020.106552. Epub 2020 Jul 22.
It is widely recognized that quality of spermatozoa in sexed semen (SS) samples is not as great as for conventional, non-sexed semen (NS). There are differences in qualitative and biochemical variables between spermatozoa in NS and SS. Information, however, is lacking on molecular differences, especially concerning spermatozoa proteomic differences is SS and NS. The objective of this study was to compare commercially available NS and SS bull semen by evaluating sperm quality variables in conjunction with a proteomics approach. Results from flow cytometry and computer-assisted sperm analyses indicated there was less sperm motility, viability, mitochondrial potential and acrosome integrity in sperm from SS. Results from proteomic analysis indicated sperm from NS and SS samples were characterized by different protein profiles. There was identification of 70 sperm proteins that differed in abundance and six protein spots that were different in extent of carbonylation. Sperm from SS had altered structures of enzymes involved in glycolysis, oxidative phosphorylation and maintenance of a constant adenylate energy charge. Furthermore, sperm from SS had alterations of several flagella substructures, especially outer dense fiber proteins, which were less abundant and more carbonylated than in sperm from NS. In sperm of SS, compared with NS, there were differences in abundance of proteins involved in capacitation, acrosome reaction and sperm-egg fusion as well as lesser abundances of sperm surface proteins. Results enable a greater understanding of differences between sperm from NS and SS samples, thereby contributing to development of improved protocols for more effective protection of sexed spermatozoa during processing.
人们普遍认为，性控精液（SS）样本中的精子质量不如常规的非性控精液（NS）。NS 和 SS 中的精子在质量和生化变量上存在差异。然而，关于分子差异的信息，特别是关于 SS 和 NS 中精子蛋白质组差异的信息还很缺乏。本研究的目的是通过评估精子质量变量并结合蛋白质组学方法，比较商业上可用的 NS 和 SS 公牛精液。流式细胞术和计算机辅助精子分析的结果表明，SS 中的精子运动性、活力、线粒体潜能和顶体完整性较低。蛋白质组学分析的结果表明，NS 和 SS 样本中的精子具有不同的蛋白质图谱。鉴定出 70 种精子蛋白的丰度存在差异，6 个蛋白斑点的羰基化程度存在差异。SS 中的精子中涉及糖酵解、氧化磷酸化和保持恒定的腺苷酸能量电荷的酶的结构发生了改变。此外，SS 中的精子的几个鞭毛亚结构发生了改变，特别是外致密纤维蛋白，其丰度低于 NS 中的精子，且羰基化程度更高。与 NS 相比，SS 中的精子在与顶体反应和精子-卵融合相关的蛋白丰度上存在差异，同时精子表面蛋白的丰度也较低。这些结果使我们更好地理解了 NS 和 SS 样本中精子之间的差异，从而有助于开发更好的保护方案，以在处理过程中更有效地保护性控精子。
