Botta A, Bruguerolle B, Bartolin R, Bouvenot G
Department of Toxicology, Occupational Health Service, Faculty of Medicine, Marseilles, France.
Chronobiol Int. 1987;4(4):509-12. doi: 10.3109/07420528709078542.
Determination of urinary delta-amino levulinic acid (ALA) is now systematically used in occupational health to detect an excessive exposure to lead in professionally exposed workers. However, to determine whether circadian changes of the urinary excretion of ALA alter the significance of the test, we quantified the ALA levels in the urine of 19 healthy young adults. Urine samples were taken every 3 hr between 0700 and 2300 hr and ALA levels were determined by a spectrocolorimetric method. The data indicated that the 24-hr mean ALA level was: 1.81 mg/g creatinine. The peak values (2.24 +/- 0.24) were obtained between 1400 and 1700 hr whereas the lowest ALA levels were found between 2200 and 0300 hr. Cosinor analysis revealed a significant circadian rhythm while no significant difference could be found according to sex. Possible explanations of our findings are discussed.
目前，职业健康领域系统地采用测定尿中δ-氨基乙酰丙酸（ALA）的方法来检测职业暴露工人是否铅接触过量。然而，为了确定ALA尿排泄的昼夜变化是否会改变该检测的意义，我们对19名健康年轻成年人尿液中的ALA水平进行了量化。在07:00至23:00之间，每3小时采集一次尿液样本，并采用分光比色法测定ALA水平。数据表明，24小时平均ALA水平为：1.81mg/g肌酐。峰值（2.24±0.24）出现在14:00至17:00之间，而最低ALA水平出现在22:00至03:00之间。余弦分析显示存在显著的昼夜节律，且按性别未发现显著差异。本文讨论了我们研究结果的可能解释。
