Tomokuni K, Ichiba M, Hirai Y, Sugimoto K, Yoshida T, Hirata M
Department of Community Health Science, Saga Medical School, Japan.
Int Arch Occup Environ Health. 1988;61(3):153-6. doi: 10.1007/BF00381011.
Fluorometric methods for determining delta-aminolevulinic acid (ALA) and coproporphyrin (CP) in urine have been recently developed by using high-performance liquid chromatography (HPLC). In the present study, urinary ALA and CP in lead-exposed workers were determined with these fluorometric HPLC methods and the conventional methods, and the results obtained were compared. In lead workers with a urinary ALA greater than or equal to 5 mg/l, the values obtained with the fluorometric HPLC method corresponded well with those measured with the conventional colorimetric method. In contrast, in lead workers with ALA less than 5 mg/l, ALA values obtained with the fluorometric HPLC method were lower than those measured with the conventional method, suggesting the possibility of matrix interference in urine. The urinary CP values obtained with the conventional method of Rimington (1971) were higher than those measured with the fluorometric HPLC method, though the correlation was good.
最近已开发出利用高效液相色谱法（HPLC）测定尿液中δ-氨基乙酰丙酸（ALA）和粪卟啉（CP）的荧光测定方法。在本研究中，采用这些荧光HPLC方法和传统方法对铅暴露工人的尿ALA和CP进行了测定，并对所得结果进行了比较。在尿ALA大于或等于5mg/l的铅作业工人中，荧光HPLC方法获得的值与传统比色法测量的值非常吻合。相比之下，在尿ALA低于5mg/l的铅作业工人中，荧光HPLC方法获得的ALA值低于传统方法测量的值，提示尿液中可能存在基质干扰。尽管相关性良好，但采用Rimington（1971年）传统方法获得的尿CP值高于荧光HPLC方法测量的值。
