Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Cells. 2021 Jul 5;10(7):1696. doi: 10.3390/cells10071696.
The creation of a testis organoid (artificial testis tissue) with sufficient resemblance to the complex form and function of the innate testis remains challenging, especially using non-rodent donor cells. Here, we report the generation of an organoid culture system with striking biomimicry of the native immature testis tissue, including vasculature. Using piglet testis cells as starting material, we optimized conditions for the formation of cell spheroids, followed by long-term culture in an air-liquid interface system. Both fresh and frozen-thawed cells were fully capable of self-reassembly into stable testis organoids consisting of tubular and interstitial compartments, with all major cell types and structural details expected in normal testis tissue. Surprisingly, our organoids also developed vascular structures; a phenomenon that has not been reported in any other culture system. In addition, germ cells do not decline over time, and Leydig cells release testosterone, hence providing a robust, tunable system for diverse basic and applied applications.
睾丸类器官（人工睾丸组织）的创建具有足够类似于先天睾丸的复杂形式和功能仍然具有挑战性，特别是使用非啮齿动物供体细胞。在这里，我们报告了一种类器官培养系统的产生，该系统具有对天然未成熟睾丸组织的惊人仿生，包括脉管系统。使用仔猪睾丸细胞作为起始材料，我们优化了细胞球体形成的条件，然后在气液界面系统中进行长期培养。新鲜和冷冻解冻的细胞都完全能够自我组装成稳定的睾丸类器官，由管状和间质隔室组成，具有正常睾丸组织中预期的所有主要细胞类型和结构细节。令人惊讶的是，我们的类器官还发育出血管结构；这一现象在任何其他培养系统中都没有报道过。此外，生殖细胞不会随时间减少，而间质细胞释放睾丸激素，因此为各种基础和应用应用提供了一个强大、可调的系统。
