Burlina A B, Goi G, Fabi A, Lombardo A, Gaburro D, Tettamanti G
Department of Paediatrics, Medical School, University of Verona, Italy.
Clin Biochem. 1987 Dec;20(6):423-7. doi: 10.1016/0009-9120(87)90009-9.
The plasma levels of three lysosomal enzymes, beta-D-N-acetylglucosaminidase, beta-D-glucuronidase, and alpha-L-fucosidase, were fluorimetrically determined in seven insulin-dependent diabetic patients one day before, one day after, and during a two-day treatment with the artificial pancreas, at 4 to 5 h intervals. A statistically significant decrease of the plasma level of each enzyme was observed during artificial pancreas treatment. The extent of decrease was 30 to 35% for beta-N-acetylglucosaminidase, 35 to 40% for beta-D-glucuronidase, and 20 to 25% for alpha-L-fucosidase. The decrease occurred earlier (at the first day of treatment) for beta-D-N-acetylglucosaminidase, and later (at the second day of treatment, and lasting to the first day after treatment) for the other two enzymes. These results suggest a direct connection between the lysosomal apparatus and insulin-controlled metabolic pathways, and a potential role for lysosomal enzymes as indicators of the metabolic compensation in diabetes.
采用荧光分析法测定了7例胰岛素依赖型糖尿病患者在人工胰腺治疗前一天、治疗后一天以及为期两天的治疗期间（每隔4至5小时）三种溶酶体酶（β-D-N-乙酰氨基葡萄糖苷酶、β-D-葡萄糖醛酸酶和α-L-岩藻糖苷酶）的血浆水平。在人工胰腺治疗期间，观察到每种酶的血浆水平均有统计学意义的下降。β-N-乙酰氨基葡萄糖苷酶的下降幅度为30%至35%，β-D-葡萄糖醛酸酶为35%至40%，α-L-岩藻糖苷酶为20%至25%。β-D-N-乙酰氨基葡萄糖苷酶的下降出现得较早（在治疗第一天），而其他两种酶的下降出现得较晚（在治疗第二天，并持续至治疗后第一天）。这些结果表明溶酶体细胞器与胰岛素控制的代谢途径之间存在直接联系，并且溶酶体酶作为糖尿病代谢补偿指标具有潜在作用。
