Finger J M, Mercer J F, Cotton R G, Danks D M
Murdoch Institute for Research into Birth Defects, Royal Children's Hospital, Parkville, Victoria, Australia.
Clin Chim Acta. 1987 Dec;170(2-3):209-18. doi: 10.1016/0009-8981(87)90130-6.
The method of two-dimensional polyacrylamide gel electrophoresis has been used to investigate the post mortem stability of protein and mRNA in human liver. The electrophoretic mobility of proteins and of the in vitro translation products of the mRNA were found to be essentially unaffected by incubation of the liver at 37 degrees C for up to 2 h or at 4 degrees C for up to 16 h. This study indicates that the major protein and mRNA species in liver are stable enough following death to allow meaningful studies on tissue collected under standard autopsy conditions.
二维聚丙烯酰胺凝胶电泳法已被用于研究人肝脏中蛋白质和mRNA的死后稳定性。研究发现，将肝脏在37℃孵育长达2小时或在4℃孵育长达16小时，蛋白质和mRNA的体外翻译产物的电泳迁移率基本不受影响。这项研究表明，肝脏中的主要蛋白质和mRNA种类在死后足够稳定，能够对在标准尸检条件下采集的组织进行有意义的研究。
