Kumar V L, Majumder P K, Murty O P, Kumar V
Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi.
Int Urol Nephrol. 1998;30(3):301-4. doi: 10.1007/BF02550313.
RNA isolated from frozen human postmortem prostate tissue was evaluated for its utility in molecular biological studies based on RNA analysis. Our results on slot-blot analysis show the presence of receptor transcripts for androgen, epidermal growth factor and basic fibroblast growth factor in postmortem prostate tissue obtained 20-120 hrs after death. However, the RNA in these samples was found to be degraded as revealed by the absence of ribosomal bands on gel electrophoresis. AR mRNA was found to be present in one of the five samples when analysed by northern blotting.
对从冷冻的人类死后前列腺组织中分离出的RNA，基于RNA分析评估其在分子生物学研究中的效用。我们斑点印迹分析的结果显示，在死亡后20 - 120小时获得的死后前列腺组织中存在雄激素、表皮生长因子和碱性成纤维细胞生长因子的受体转录本。然而，如凝胶电泳中核糖体条带的缺失所示，这些样本中的RNA被发现已降解。通过Northern印迹分析时，在五个样本中的一个样本中发现了AR mRNA。
