School of Energy Sciences and Engineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
School of Energy Sciences and Engineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India; Carbohydrate Enzyme Biotechnology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
Bioresour Technol. 2022 May;351:127057. doi: 10.1016/j.biortech.2022.127057. Epub 2022 Mar 23.
Deep eutectic solvent comprising choline chloride (ChCl) and acetic acid (AA) was used for rice straw (RS) pretreatment. Effect of ChCl: AA molar ratio, time and temperature on lignin removal and retainment of total carbohydrate content (TCC) in pretreatment process were evaluated by central composite design (CCD) approach. The pretreatment temperature and molar ratio of AA to ChCl played a significant role in delignification. The optimized conditions for RS pretreatment were 1:3.59 (ChCl:AA molar ratio), 126 °C and 150 min. ChCl:AA pretreated RS (CApRS) gave 83.1% delignification, 679 mg/g TCC and 83.7% pretreatment efficiency. CApRS contained enriched cellulose content, 0.73 g/g as compared with 0.43 g/g in raw RS. CApRS showed 31% higher crystallinity index, 17-fold higher surface area than raw RS. The morphological study of CApRS displayed porous surface. Saccharification of CApRS by commercial cellulase gave total reducing sugar of 18.8 g/L in hydrolysate with saccharification efficiency, 92.2%.
包含胆碱氯化物 (ChCl) 和乙酸 (AA) 的深共熔溶剂用于稻秆 (RS) 预处理。通过中心复合设计 (CCD) 方法评估了 ChCl:AA 摩尔比、时间和温度对预处理过程中木质素去除和总碳水化合物含量 (TCC) 保留的影响。预处理温度和 AA 与 ChCl 的摩尔比对脱木质素起着重要作用。RS 预处理的最佳条件为 1:3.59（ChCl:AA 摩尔比）、126°C 和 150min。ChCl:AA 预处理的 RS (CApRS) 脱木质素率为 83.1%，TCC 为 679mg/g，预处理效率为 83.7%。CApRS 含有丰富的纤维素含量，为 0.73g/g，而原 RS 为 0.43g/g。CApRS 的结晶度指数比原 RS 高 31%，比表面积高 17 倍。CApRS 的形态研究显示出多孔表面。商用纤维素酶对 CApRS 的糖化产生了水解液中 18.8g/L 的总还原糖，糖化效率为 92.2%。
