Department of Chemical Engineering, Faculty of Engineering, Kermanshah University of Technology, Kermanshah, Iran.
Department of Chemical Engineering, Faculty of Engineering, Kermanshah University of Technology, Kermanshah, Iran.
Talanta. 2021 Nov 1;234:122698. doi: 10.1016/j.talanta.2021.122698. Epub 2021 Jul 9.
The MWCNTs/FeO@TMU-21 as a novel electrochemical sandwich-type genosensor was fabricated to detect the BRCA1 gene using the redox-cycling ferrocene functionalized reporter label probe (r-Fc-DNA). In the designed genosensor, the capture probe (cDNA) and r-Fc-DNA were used to detect the BRCA1 gene in sandwich-type genosensor, in which DNA sequences are well -hybridized with the BRCA1 gene (t-DNA). The cDNA was immobilized on the multiwall carbon nanotube and metal-organic framework with FeO nanoparticle core, which is the sensor platform. Target DNA was assayed by redox-recycling reporter probe (r-Fc-DNA) using the electro-catalytic activity of ferri/ferrocyanide, which results in significantly enhanced the oxidation peak current of r-Fc-DNA. The electrochemical redox cycling led to a high signal-to-noise ratio for gene assay. MWCNTs and FeO@TMU-21 were applied to increase the platform conductivity and suitable binding of the recognition elements. This constructed genosensor plays an influential role in increasing the sensitivity of BRCA1 gene sequence recognition. So that under optimal conditions, this genosensor illustrated a wide linear range from 1.0×10 to 1.0×10 M with a detection limit of 0.57 × 10 M. Moreover, the genosensor exhibited high selectivity, stability, and reproducibility. The obtained recoveries (between 91 and 105%) of the BRCA1 gene assay in human blood samples satisfactory, which can be used for BRCA1 gene measurement in the laboratory.
MWCNTs/FeO@TMU-21 作为一种新型电化学三明治型基因传感器，用于检测 BRCA1 基因，使用的是循环伏安铁功能化报告标签探针（r-Fc-DNA）。在设计的基因传感器中，捕获探针（cDNA）和 r-Fc-DNA 用于三明治型基因传感器中检测 BRCA1 基因，其中 DNA 序列与 BRCA1 基因（t-DNA）很好地杂交。cDNA 固定在多壁碳纳米管和金属有机骨架上，具有 FeO 纳米颗粒核，作为传感器平台。目标 DNA 通过循环伏安铁/亚铁氰化物的电催化活性，利用报告探针（r-Fc-DNA）进行测定，从而显著增强 r-Fc-DNA 的氧化峰电流。电化学循环伏安导致基因检测的信号与噪声比显著提高。MWCNTs 和 FeO@TMU-21 被应用于增加平台导电性和识别元件的适当结合。这种构建的基因传感器在增加 BRCA1 基因序列识别的灵敏度方面发挥了重要作用。因此，在最佳条件下，该基因传感器显示出从 1.0×10 到 1.0×10 M 的宽线性范围，检测限为 0.57×10 -6 M。此外，该基因传感器表现出高选择性、稳定性和重现性。在人血样中进行 BRCA1 基因检测的回收率（在 91%至 105%之间）令人满意，可用于实验室中 BRCA1 基因的测量。
