Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Jiangsu Key Laboratory of Oral Diseases, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, Jiangsu, China.
Carbohydr Polym. 2021 Oct 15;270:118342. doi: 10.1016/j.carbpol.2021.118342. Epub 2021 Jun 21.
The naturally tight entanglement of fibers in bacterial cellulose (BC) results in low printability when BC is used as a bioink for printing scaffolds. In this study, neat BC was treated by TEMPO-mediated oxidation (TO-BC) and maleic acid (MA-BC) to prepare homogeneous BC dispersions to fabricate scaffolds for bone regeneration. Results showed that the treatments released individual fibrils in the corresponding uniform dispersions without impairing inherent crystalline properties. Compared with TO-BC, MA-BC hybridized with gelatin could endow the gel with improved rheological properties and compression modulus for 3D printing. Both TO-BC and MA-BC dispersions showed good osteoblast viability. However, MA-BC possessed more pronounced ability to express osteogenic marker genes and formation of mineralized nodules in vitro. Compared with TO-BC-based gelatin scaffolds, MA-BC-based gelatin scaffolds showed a better ability to stimulate the regeneration of rat calvaria, demonstrating a higher bone mineral density of newly formed bone and trabecular thickness in vivo.
细菌纤维素（BC）中的纤维天然紧密缠绕，导致其作为生物墨水打印支架时印刷性能不佳。在这项研究中，通过 TEMPO 介导的氧化（TO-BC）和马来酸（MA-BC）处理来制备均匀的 BC 分散体，以制造用于骨再生的支架。结果表明，这些处理方法在相应的均匀分散体中释放出了单个纤维，而不会损害其固有结晶性能。与 TO-BC 相比，与明胶复合的 MA-BC 可以赋予凝胶更好的流变性能和压缩模量，适用于 3D 打印。TO-BC 和 MA-BC 分散体均显示出良好的成骨细胞活力。然而，MA-BC 在外显子表达成骨标志物基因和矿化结节形成方面表现出更显著的能力。与基于 TO-BC 的明胶支架相比，基于 MA-BC 的明胶支架在刺激大鼠颅骨再生方面表现出更好的能力，体内新形成骨和小梁厚度的骨矿物质密度更高。
