Transcriptome analysis of differentially expressed genes in chrysanthemum RNA interference lines.

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DNA methylation is the most important epigenetic modification involved in many essential biological processes. is one of DNA methyltransferases that affect the level of methylation in the entire genome. To explore the effect of gene silencing on gene expression profile of  ×  'Zijingling'. The stem section and leaves at the young stage were taken for transcriptome sequencing. - leaves had 8 differentially expressed genes while 156 differentially expressed genes were observed in - stem compared with control leaves and stem. These genes encode many key proteins in plant biological processes, such as transcription factors, signal transduction mechanisms, secondary metabolite synthesis, transport and catabolism and interaction. In general, 34.58% of the differentially expressed genes in leaves and stems were affected by the reduction of the gene. The differentially expressed genes in stem and leaves of transgenic plants went through significant changes. We found adequate amount of candidate genes associated with flowering, however, the number of genes with significant differences between transgenic and control lines was not too high. Several flowering related genes were screened out for gene expression verification and all of them were obseved as consistent with transcriptome data. These candidate genes may play important role in flowering variation of chrysanthemum. This study reveals the mechanism of interference on the growth and development of chrysanthemum at the transcriptional level, which provides the basis for further research on the epigenetic regulation mechanism in flower induction and development.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s12298-021-01022-1.