The DmsABC Sulfoxide Reductase Supports Virulence in Non-typeable .

Although molybdenum-containing enzymes are well-established as having a key role in bacterial respiration, it is increasingly recognized that some may also support bacterial virulence. Here, we show that DmsABC, a putative dimethylsulfoxide (DMSO) reductase, is required for fitness of the respiratory pathogen (Hi) in different models of infection. Expression of the operon increased with decreasing oxygen availability, but despite this, a Hi2019 strain did not show any defects in anaerobic growth on chemically defined medium (CDM), and viability was also unaffected. Although Hi2019 exhibited increased biofilm formation and greater resistance to hypochlorite killing compared to the isogenic wild-type strain, its survival in contact with primary human neutrophils, in infections of cultured tissue cells, or in a mouse model of lung infection was reduced compared to Hi2019. The tissue cell infection model revealed a two-fold decrease in intracellular survival, while in the mouse model of lung infection Hi2019 was strongly attenuated and below detection levels at 48 h post-inoculation. While Hi2019 was recovered in approximately equal numbers from bronchoalveolar lavage fluid (BALF) and lung tissue, survival of Hi2019 was reduced in lung tissue compared to BALF samples, indicating that Hi2019 had reduced access to or survival in the intracellular niche. Our data clearly indicate for the first time a role for DmsABC in infection and that the conditions under which DmsABC is required in this bacterium are closely linked to interactions with the host.