Genesis Research Services, Broadmeadow, NSW, Australia.
Centre for Inflammatory Diseases, School of Clinical Sciences at Monash Health, Monash University, Clayton, VIC, Australia.
Front Immunol. 2021 Jul 23;12:708425. doi: 10.3389/fimmu.2021.708425. eCollection 2021.
Interleukin (IL)-37 has an important function in limiting excessive inflammation. Its expression is increased in numerous inflammatory and autoimmune conditions and correlates with disease activity, suggesting it could have potential as a disease biomarker. Nevertheless, a reference range has yet to be determined. Our aim was to establish the first reference range of circulating IL-37 levels in healthy adult humans. PubMed was searched for studies reporting blood IL-37 concentrations in healthy adult subjects as measured by enzyme-linked immunosorbent assay. Nineteen studies were included in the analysis. Mean IL-37 levels were weighted by sample sizes, and weighted mean lower and upper levels ( ± 2SD of means) were calculated to provide a weighted mean and reference range. IL-37 levels were quantified in either serum or plasma from a total of 1035 (647 serum; 388 plasma) healthy subjects. The serum, plasma and combined matrix weighted means (reference ranges) were 72.9 (41.5 - 104.4) pg/mL, 83.9 (41.1 - 126.8) pg/mL, and 77.1 (41.4 - 112.8) pg/mL, respectively. There were no significant differences between serum and plasma means and upper and lower limits. Study means and upper IL-37 levels were significantly higher in Chinese population studies. From our analysis, a preliminary reference range for circulating IL-37 levels in healthy human adults has been established. In order to determine a reliable reference range for clinical application, large, prospective, multi-ethnic, healthy population studies are necessary. In addition, demographics, sample matrix, collection, processing and storage methods potentially affecting IL-37 detection levels should be thoroughly investigated.
白细胞介素 (IL)-37 在限制过度炎症方面具有重要作用。它的表达在许多炎症和自身免疫性疾病中增加，并与疾病活动相关，表明它可能具有作为疾病生物标志物的潜力。然而，尚未确定参考范围。我们的目的是建立健康成年人群中循环 IL-37 水平的第一个参考范围。通过酶联免疫吸附试验检测健康成年受试者血液中 IL-37 浓度的研究，在 PubMed 上进行了检索。分析中纳入了 19 项研究。根据样本量对平均 IL-37 水平进行加权，计算加权平均下限和上限（均值的 ± 2SD），以提供加权平均值和参考范围。从总共 1035 名（647 份血清；388 份血浆）健康受试者的血清或血浆中定量了 IL-37 水平。血清、血浆和组合基质加权平均值（参考范围）分别为 72.9（41.5-104.4）pg/mL、83.9（41.1-126.8）pg/mL 和 77.1（41.4-112.8）pg/mL。血清和血浆平均值以及上限和下限之间无显著差异。中国人群研究的研究平均值和 IL-37 上限明显较高。通过我们的分析，已经为健康成年人群中循环 IL-37 水平建立了初步的参考范围。为了确定用于临床应用的可靠参考范围，需要进行大型、前瞻性、多民族、健康人群研究。此外，应彻底研究可能影响 IL-37 检测水平的人口统计学、样本基质、采集、处理和储存方法。
