Barug Daan, Goorden Susan, Herruer Martien, Müller Moira, Brohet Richard, de Winter Peter
Department of Pediatrics, Spaarne Hospital, Hoofddorp, The Netherlands.
Atal-Medial Medical Diagnostic Centers, Hoofddorp, The Netherlands.
PLoS One. 2014 Dec 8;9(12):e114109. doi: 10.1371/journal.pone.0114109. eCollection 2014.
Automated interleukin assays are promising diagnostic aids for early-onset neonatal sepsis, however, reference values for healthy term neonates are incompletely known. The goal of this study is to determine reference values for interleukin-6 (IL-6) and interleukin-8 (IL-8) in cord blood of healthy term neonates.
Women were recruited from April 2012 to August 2012. IL-6 and IL-8 levels were measured using an automated immunometric assay (Immulite) in cord blood of 93 healthy term newborns, 60 of them were born via vaginal delivery and 33 by elective caesarean section (ECS). A mean value for IL-8 of 8.1 ± 3.0 pg/mL was found in cord blood of healthy term neonates, which apply to both vaginal delivery and ECS. Regarding IL-6, two values apply. For vaginal delivery, a median value of 3.3 pg/mL (range, <2 to 9.53 pg/mL) was found, while for ECS, a median value of <2 pg/mL (range, <2 to 48 pg/mL) applies.
We propose a reference value of <14.1 pg/mL for IL-8 (mean + 2SD), applying to vaginally delivered and ECS-delivered healthy term newborns. From a clinical point of view, we also propose one reference value for IL-6 to be applied to vaginally delivered and ECS-delivered healthy term newborns, which is <10.2 pg/mL (97.5th percentile total group). These values have to be validated in larger cohorts of neonates, inclusive of those with and without early-onset neonatal sepsis.
自动化白细胞介素检测对于早发型新生儿败血症是很有前景的诊断辅助手段，然而，健康足月儿的参考值尚不完全清楚。本研究的目的是确定健康足月儿脐带血中白细胞介素-6（IL-6）和白细胞介素-8（IL-8）的参考值。
2012年4月至2012年8月招募了一些女性。使用自动化免疫分析方法（免疫发光分析仪）对93名健康足月儿的脐带血进行IL-6和IL-8水平检测，其中60名经阴道分娩，33名经择期剖宫产（ECS）。在健康足月儿脐带血中发现IL-8的平均值为8.1±3.0 pg/mL，这适用于阴道分娩和择期剖宫产。关于IL-6，有两个值适用。对于阴道分娩，发现中位数为3.3 pg/mL（范围，<2至9.53 pg/mL），而对于择期剖宫产，适用的中位数为<2 pg/mL（范围，<2至48 pg/mL）。
我们提出IL-8的参考值<14.1 pg/mL（均值 + 2标准差），适用于经阴道分娩和择期剖宫产分娩的健康足月儿。从临床角度来看，我们还提出一个适用于经阴道分娩和择期剖宫产分娩的健康足月儿的IL-6参考值，即<10.2 pg/mL（全组第97.5百分位数）。这些值必须在更大的新生儿队列中进行验证，包括有和没有早发型新生儿败血症的新生儿。
